Concept

Cell counting

Publications associées (33)

Modeling, Simulation, and Performance Evaluation of a Novel Microfluidic Impedance Cytometer for Morphology-Based Cell Discrimination

Philippe Renaud, Ludovica Colella, Marjan Shaker

The performance of a novel microfluidic impedance cytometer [1] for single-cell analysis is investigated in-silico by means of a finite element model. The main feature of the device is the ability to probe impedance of flowing cells along two orthogonal di ...

Ieee-Inst Electrical Electronics Engineers Inc2014

This paper reports a novel impedance cytometer design, easily integrable with dielectrophoretic focusing using a simple fabrication process with a single metal layer. Patterning of electrodes recessed in lateral channels – so-called “liquid electrodes” – a ...

Royal Soc Chemistry2012

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Counting cells in a large microchannel remains challenging and is particularly critical for in vitro assays, such as cell adhesion assays. This paper addresses this issue, by presenting the development of interdigitated three-dimensional electrodes, which ...

Ieee2012

Automated analysis of single stem cells in microfluidic traps

Matthias Lütolf, Alessandra Griffa, Olivier Burri, Arne Seitz, Stefan Kobel, Mukul Girotra

We report a reliable strategy to perform automated image cytometry of single (non-adherent) stem cells captured in microfluidic traps. The method rapidly segments images of an entire microfluidic chip based on the detection of horizontal edges of microflui ...

2012

On chip cellular dielectric spectroscopy

Ludovica Colella

In the recent years, the development of automated systems for cellular monitoring has been highly promoted in basic biological research as well as in industries and medical diagnostic. Several methods for transduction of cellular signals have been investig ...

EPFL2012

Cell viability assessment by flow cytometry using yeast as cell model

Philippe Renaud, Niccolo Piacentini, Raphaël Tornay, Nina Buffi

This paper reports the new combination of cell sorting and counting capabilities on a single device. Most state-of-the-art devices combining these technologies use optical techniques requiring complicated experimental setups and labeled samples. The use of ...

2011

Targeting of multiple metabolites in neural cells monitored by using protein-based carbon nanotubes

Giovanni De Micheli, Sandro Carrara, Cristina Boero, Giovanna Del Vecchio

Microdevices dedicated to monitor metabolite levels have recently enabled many applications in the field of cell analysis, to monitor cell growth and development of numerous cell lines. By combining the traditional technology used for electrochemical biose ...

2011

Multiple-frequency Impedance Measurements in Continuous Flow for the Evaluation of Electrical Lysis of Yeast Cells

Philippe Renaud, Niccolo Piacentini, Willyan Hasenkamp Carreira

his paper reports on an electrical lysis device with integrated cell counting and evaluation of the lysis efficiency by multiplefrequency impedance measurements. Conventional techniques use chemical reagents to lyse cells, causing unwanted interference wit ...

Elsevier2010

Development of high content image-based cell viability assays

Julien Mena

Aim and applications: 1. Optimize fluorescence-based cellular assays for getting insights about mechanisms leading to cell death 2. Informative high content assays development with the aim to implement them in order to obtain preliminary toxicity informati ...

2010

Individual cells immobilization for water-borne pathogen detection and enumeration

A cell counting device has been proposed and implemented for water-borne pathogen detection for drinking water quality monitoring applications. Our approach is based on magnetically-labelled cells immobilization in a high density array of individual cell f ...

Elsevier Science, Reg Sales Off, Customer Support Dept, 655 Ave Of The Americas, New York, Ny 10010 Usa2009

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