Concept

Mating of yeast

Publications associées (119)

The S-pombe cytokinesis NDR kinase Sid2 activates Fin1 NIMA kinase to control mitotic commitment through Pom1/Wee1

Mitotic exit integrates the reversal of the phosphorylation events initiated by mitotic kinases with a controlled cytokinesis event that cleaves the cell in two. The mitotic exit network (MEN) of budding yeast regulates both processes, whereas the fission ...

Nature Publishing Group2012

Production of a thermostable fumarase in Saccharomyces cerevisiae for the bioconversion of fumarate to L-malate

Coralie Signorell

The gene encoding fumarase (fum) from Thermus thermophilus was expressed in yeast Saccharomyces cerevisiae. The recombinant cells were heated at 70°C to inactivate indigenous enzymes and used for the bioconversion of fumaric acid to L-malic acid. By heatin ...

2011

The PIAS homologue Siz2 regulates perinuclear telomere position and telomerase activity in budding yeast

Joachim Lingner

Budding yeast telomeres are reversibly bound at the nuclear envelope through two partially redundant pathways that involve the Sir2/3/4 silencing complex and the Yku70/80 heterodimer(1,2). To better understand how this is regulated, we studied the role of ...

2011

Dividing the spoils of growth and the cell cycle: The fission yeast as a model for the study of cytokinesis

Viesturs Simanis, Anupama Goyal

Cytokinesis is the final stage of the cell cycle, and ensures completion of both genome segregation and organelle distribution to the daughter cells. Cytokinesis requires the cell to solve a spatial problem (to divide in the correct place, orthogonally to ...

2011

Tracking and synchronization of the yeast cell cycle using dielectrophoretic opacity

Philippe Renaud, Thomas Michael Braschler, Nicolas Demierre, Ana Valero

Cell cycle synchronization is an important tool for the study of the cell division stages and signalling. It provides homogeneous cell cultures that are of importance to develop and improve processes such as protein synthesis and drug screening. The main a ...

Royal Society of Chemistry2011

Multiple-frequency Impedance Measurements in Continuous Flow for the Evaluation of Electrical Lysis of Yeast Cells

Philippe Renaud, Niccolo Piacentini, Willyan Hasenkamp Carreira

his paper reports on an electrical lysis device with integrated cell counting and evaluation of the lysis efficiency by multiplefrequency impedance measurements. Conventional techniques use chemical reagents to lyse cells, causing unwanted interference wit ...

Elsevier2010

The role of Schizosaccharomyces pombe dma1 in spore formation during meiosis

Viesturs Simanis, Andrea Krapp, Elena Cano

Meiosis is a specialised form of the cell cycle that gives rise to haploid gametes. In Schizosaccharomyces pombe, the products of meiosis are four spores, which are formed by encapsulation of the four meiosis II nuclei within the cytoplasm of the zygote pr ...

2010

A miniaturized continuous dielectrophoretic cell sorter and its applications

Philippe Renaud, Thomas Michael Braschler, Nicolas Demierre, Ana Valero

There is great interest in highly sensitive separation methods capable of quickly isolating a particular cell type within a single manipulation step prior to their analysis. We present a cell sorting device based on the opposition of dielectrophoretic forc ...

American Institute of Physics2010

Task-dependent influence of genetic architecture and mating frequency on division of labour in social insect societies

Dario Floreano, Laurent Keller

Division of labour is one of the most prominent features of social insects. The efficient allocation of individuals to different tasks requires dynamic adjustment in response to environmental perturbations. Theoretical models suggest that the colony-level ...

Springer Verlag2010

Stringent requirement for HRD1, SEL1L, and OS-9/XTP3-B for disposal of ERAD-L-S substrates

Maurizio Molinari, Riccardo Bernasconi

Sophisticated quality control mechanisms prolong retention of protein-folding intermediates in the endoplasmic reticulum (ER) until maturation while sorting out terminally misfolded polypeptides for ER-associated degradation (ERAD). The presence of structu ...

2010

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