Concept

Mating of yeast

Publications associées (119)

GTPase Activity Plays a Key Role in the Pathobiology of LRRK2

Mutations in the leucine-rich repeat kinase 2 (LRRK2) gene are associated with late-onset, autosomal-dominant, familial Parkinson's disease (PD) and also contribute to sporadic disease. The LRRK2 gene encodes a large protein with multiple domains, includin ...

Public Library of Science2010

Label-free Sorting and Counting of Yeast Cells for Viability Studies

Philippe Renaud, Niccolo Piacentini, Raphaël Tornay, Nina Buffi

This paper reports the new combination of cell sorting and counting capabilities on a single device. Most state-of-the-art devices combining these technologies use optical techniques requiring complicate experimental setups and labeled samples. The use of ...

Elsevier2009

Use of self-actuating and self-sensing cantilevers for imaging biological samples in fluid

Georg Fantner

In this paper, we present a detailed investigation into the suitability of atomic force microscopy (AFM) cantilevers with integrated deflection sensor and micro-actuator for imaging of soft biological samples in fluid. The Si cantilevers are actuated using ...

2009

Integration of plasmonic trapping in a microfluidic environment

Olivier Martin, Sebastian Maerkl, Lina Huang

Near field generated by plasmonic structures has recently been proposed to trap small objects. We report the first integration of plasmonic trapping with microfluidics for lab-on-a-chip applications. A three-layer plasmo-microfluidic chip is used to demons ...

Optical Society of America2009

Cytokinesis: Closure resets your SIN

Viesturs Simanis, Andrea Krapp, Evelyn Lattmann

A new study of fission yeast cell division has revealed a coupling between cytoplasmic partitioning and the turning-off of cytokinesis signalling that may be mediated by asymmetric protein distribution. ...

2009

Functional differentiation of tbf1 orthologues in fission and budding yeasts

Viesturs Simanis, Elena Cano

In Saccharomyces cerevisiae, TBF1, an essential gene, influences telomere function but also has other roles in the global regulation of transcription. We have identified a new member of the tbf1 gene family in the mammalian pathogen Pneumocystis carinii. W ...

2009

Dielectrophoresis-based continuous-flow particle microreactor

Raphaël Tornay

This thesis mainly deals with the integration of a microfluidic system for the extraction of micrometric and submicrometric particles from a solution. Traditional methods take from several minutes to hours to separate particles from a solution. Microfluidi ...

EPFL2009

Dynamic modeling of the TOR signaling pathway in yeast

Irina Krier

This Master thesis presents a complete cycle in the use of models for the study of a complex pathway like the TOR pathway. Previous models were reproduced for the phosphatase part of the pathway, and the assumptions used and the results they provided were ...

2009

Plants, MEN and SIN

Viesturs Simanis

In fission yeast, the onset of septation is signalled through the septum initiation network (SIN) signaling pathway. Similarly, in budding yeast the onset of budding is signalled through the mitotic exit network (MEN) pathway. We previously characterized i ...

2008

Focusing and continuous separation of cells in a microfluidic device using lateral dielectrophoresis

Philippe Renaud, Thomas Michael Braschler, Nicolas Demierre, Randoald Müller

We have fabricated a microfluidic device based on a novel geometrical arrangement of lateral metal electrodes and a patterned insulator. Our device combines the concept of insulator-based “electrodeless” dielectrophoresis with multiple frequencies to achie ...

2008

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