Concept

Cloning vector

Publications associées (130)

Imaging of G protein-coupled receptors in solid- supported planar membranes at the single molecule level

Odorant receptors are an excellent example of natural superiority in specifically binding specific, small and hydrophobic molecules. They are of particular interest in the development of a sensor platform for G protein- coupled receptors (GPCRs). Odorant r ...

SPIE2008

Direct Reprogramming of Terminally Differentiated Mature B Lymphocytes to Pluripotency

Patrick Schorderet

The therapeutic potential of embryonic stem cells (ESc) is a key scientific interest, but the ethical implications of stem cell research have been controversial and are often a major detriment to the advancement of our knowledge. Recently, several landmark ...

2008

Metabolic correction in oligodendrocytes derived from metachromatic leukodystrophy mouse model by using encapsulated recombinant myoblasts

Patrick Aebischer

In an effort to develop an encapsulated cell-based system to deliver arylsulfatase A (ARSA) to the central nervous system of metachromatic leukodystrophy (MLD) patients, we engineered C2C12 mouse myoblasts with a retroviral vector containing a full-length ...

2007

Genetic diversity of cyanobacterial communities in Lake Kinneret (Israel) using 16S rRNA gene, psbA and ntcA sequence analyses

The genetic diversity of cyanobacterial communities was studied at various depths in Lake Kinneret (Israel). Denaturing gradient gel electrophoresis (DGGE) of specific 16S rRNA gene PCR products showed significant differences in the cyanobacterial communit ...

2007

Inhibition of HIV-1 multiplication by a modified U7 snRNA inducing Tat and Rev exon skipping

Didier Trono

The HIV-1 regulatory proteins Tat and Rev are encoded by multiply spliced mRNAs that differ by the use of alternative 3' splice sites at the beginning of the internal exon. If these internal exons are skipped, the expression of these genes, and hence HIV-1 ...

Wiley-Blackwell2007

High-titer, serum-free production of adeno-associated virus vectors by polyethyleneimine-mediated plasmid transfection in mammalian suspension cells

Florian Maria Wurm, Lucia Baldi Unser, Mathieu Benjamin Stettler

Adeno-associated virus (AAV)-based vectors belong to the most promising gene transfer vectors in clinical studies. To provide vector for late-stage clinical trials as well as for a potential commercial phase, a scalable, cGMP-compliant process is required. ...

2007

High-titer, serum-free production of adeno-associated virus vectors by polyethyleneimine-mediated plasmid transfection in mammalian suspension cells

Florian Maria Wurm, Lucia Baldi Unser, Mathieu Benjamin Stettler

Springer, Springer Netherlands2007

Subspace properties of randomized network coding

Christina Fragouli, Suhas Diggavi, Mahdi Jafari Siavoshani

Randomized network coding has network nodes randomly combine and exchange linear combinations of the source packets. A header appended to the packet, called coding vector, specifies the exact linear combination that each packet carries. The main contributi ...

2007

Role of the methylcitrate cycle in propionate metabolism and detoxification in Mycobacterium smegmatis

John McKinney

Catabolism of odd-chain-length fatty acids yields acetyl-CoA and propionyl-CoA. A common pathway of propionyl-CoA metabolism in micro-organisms is the methylcitrate cycle, which includes the dedicated enzymes methylcitrate synthase (MCS), methylcitrate deh ...

2007

Generation of recombinant Chinese hamster ovary cell lines by microinjection

Florian Maria Wurm, Madiha Derouazi, Martin Jordan, Philippe Pierre Girard

Microinjection is a gene transfer technique enabling partial control of plasmid delivery into the nucleus or cytoplasm of cultured animal cells. Here this method was used to establish various recombinant mammalian cell lines. The injection volume was estim ...

Kluwer Academic Publishers, Springer2006

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