Concept
Phenotypic screening
Publications associées (51)
Deconvolution of ex-vivo drug screening data and bulk tissue expression predicts the abundance and viability of cancer cell subpopulations
Ex-vivo drug sensitivity screening (DSS) allows the prediction of cancer treatment effectiveness in a personalized fashion. However, it only provides a readout on mixtures of cells, potentially occulting important information on clinically relevant cell su ...
EPFL2024Drug Screening in C. elegans to identify new pharmacological mitochondrial stress inducers
Mitochondria are essential organelles participating in numerous cellular functions, including energy harvesting, regulation of homeostasis and apoptosis. Changes in mitochondrial number, morphology, and function not only impact cellular metabolism but also ...
EPFL2024A multi-reservoir extruder for time-resolved serial protein crystallography and compound screening at X-ray free-electron lasers
Christoph Bostedt, Camila Bacellar Cases Da Silveira
Serial crystallography at X-ray free-electron lasers (XFELs) permits the determination of radiation-damage free static as well as time-resolved protein structures at room temperature. Efficient sample delivery is a key factor for such experiments. Here, we ...
Berlin2023The predictive capacity of in vitro preclinical models to evaluate drugs for the treatment of retinoblastoma
Paul Joseph Dyson, Irina Sinenko, Roland Christopher Turnell-Ritson
Retinoblastoma is a rare childhood cancer of the eye. Of the small number of drugs are used to treat retinoblastoma, all have been repurposed from drugs developed for other conditions. In order to find drugs or drug combinations better suited to the improv ...
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD2023Method for hormone or drug screening in a tissue sample
Cathrin Brisken, Christoph Merten, Yueyun Zhang
The present invention relates to a method for analyzing cellular responsiveness to hormones or drags in a tissue sample based on an alginate-based 3D ex vivo culture system for normal and malignant tissue microstructures and uses thereof. ...
2023Novel High-Throughput Fluorescence-Based Assay for the Identification of Nematocidal Compounds That Target the Blood-Feeding Pathway
Gerardo Turcatti, Marc Chambon, Anthony Marchand, Nicola Harris, Tiffany Babette Angélique Bouchery, Mati André Marie Moyat
Hookworm infections cause a neglected tropical disease (NTD) affecting ~740 million people worldwide, principally those living in disadvantaged communities. Infections can cause high morbidity due to their impact on nutrient uptake and their need to feed o ...
2022Monitoring alpha-synuclein oligomerization and aggregation using bimolecular fluorescence complementation assays: What you see is not always what you get
Hilal Lashuel, Nathan Alain Denis Riguet, Bryan Frey, Abdelrahman Mohammed Adel Elsaeed Mansour AlOkda
Bimolecular fluorescence complementation (BiFC) was introduced a decade ago as a method to monitor alpha-synuclein (alpha-syn) oligomerization in intact cells. Since then, several alpha-syn BiFC cellular assays and animal models have been developed based o ...
2020Characterization of the Mercapturic Acid Pathway, an Important Phase II Biotransformation Route, in a Zebrafish Embryo Cell Line
Kristin Schirmer, Alena Tierbach
In view of the steadily increasing number of chemical compounds used in various products and applications, high-throughput toxicity screening techniques can help meeting the needs of 21st century risk assessment. Zebrafish (Danio rerio), especially its ear ...
AMER CHEMICAL SOC2020Automated Platform for Long-Term Culture and High-Content Phenotyping of Single C. elegans Worms
Johan Auwerx, Martinus Gijs, Matteo Cornaglia, Laurent Mouchiroud, Elena Katsyuba, Hüseyin Baris Atakan, Rongrong Xiang
The nematode Caenorhabditis elegans is a suitable model organism in drug screening. Traditionally worms are grown on agar plates, posing many challenges for long-term culture and phenotyping of animals under identical conditions. Microfluidics allows for ' ...
2019A Universal Assay for Aminopeptidase Activity and Its Application for Dipeptidyl Peptidase-4 Drug Discovery
Gerardo Turcatti, Marc Chambon, Julien Bortoli Chapalay, Elena Goun, Jonathan Patrice Vesin, Arkadiy Bazhin, James William Collins
Aminopeptidases, such as dipeptidyl peptidase 4 (DPP-4, CD26), are potent therapeutic targets for pharmacological interventions because they play key roles in many important pathological pathways. To analyze amino-peptidase activity in vitro (including hig ...
AMER CHEMICAL SOC2019