Concept

Neurorécepteur

Publications associées (136)

Investigating cellular signalling at the nanometer and attoliter scale

Spatial compartmentalisation is a prerequisite for the creation of living matter. Without the existence of clearly defined borders, differentiation and diversity at the cellular level would not be possible. Most scientific disciplines that deal with dissol ...

2005

Noninvasive imaging of 5-HT3 receptor trafficking in live cells: From biosynthesis to endocytosis

Horst Vogel, Horst Pick, Cédric Deluz, Erwin Ilegems

Sequential stages in the life cycle of the ionotropic 5-HT3 receptor (5-HT3R) were resolved temporally and spatially in live cells by multicolor fluorescence confocal microscopy. The insertion of the enhanced cyan fluorescent protein into the large intrace ...

2004

In vivo protein labeling for structural and functional investigation of the 5-HT3A neurotransmitter receptor

Erwin Ilegems

In this thesis, two different fluorescent labeling techniques for in vivo investigations on the 5-HT3 receptor (5-HT3R) functions are presented. This plasma membrane protein contains five subunits surrounding an ion channel that opens after binding of a 5- ...

EPFL2004

Native membranes on subwavelentgh holes for membrane receptors analysis

Jürgen Brugger, Benjamin André René Meyer

How membrane receptors transduce external signals across the membrane and activate intracellular processes is a key question for fundamental research and for therapeutic purposes. Reliable in situ investigations requiest to preserve the full biological act ...

2004

Regulation of brain proteolytic activity is necessary for the in vivo function of NMDA receptors

Carl Petersen, Sandrine Lefort

Serine proteases are considered to be involved in plasticity-related events in the nervous system, but their in vivo targets and the importance of their control by endogenous inhibitors are still not clarified. Here, we demonstrate the crucial role of a po ...

2004

Reversible site-selective labeling of membrane proteins in live cells

Horst Vogel, Rudolf Hovius, Emmanuel Guignet

Chem. and biol. labeling is fundamental for the elucidation of the function of proteins within biochem. cellular networks. In particular, fluorescent probes allow detection of mol. interactions, mobility and conformational changes of proteins in live cells ...

2004

Morphological and molecular heterogeneity in release sites of single neurons

Harald Hirling, Juan-Carlos Sarria, Xin Wang

We have previously shown that labelling intensities for synaptic proteins vary strongly among synaptic boutons. Here we addressed the questions as to whether there are heterogeneous levels of integral membrane synaptic vesicle proteins at distinct active r ...

2003

Monitoring expression and clustering of the ionotropic 5HT3 receptor in plasma membranes of live biological cells

Horst Vogel, Rudolf Hovius, Horst Pick, Michael Mayer

The ionotropic 5HT3 receptor was expressed in transiently transfected mammalian cells, yielding an unprecedented high concn. of up to 12 million receptors per cell. Receptor traffic in the plasma membrane of live cells was obsd. continuously over 24 h by f ...

2003

Stabilization of molecular LiF, LiFHF, and Na2SiF6 using metallamacrocyclic hosts

Kay Severin

The molecular forms of LiF, LiFHF and Na2SiF6 have been stabilized using trinuclear metallamacrocyclic complexes of (cymene)Ru-II, (Cp*)Rh-III and (Cp*)Ir-III as specific receptors. The host-guest complexes were characterized by NMR spectroscopy and single ...

2003

Cellular perspectives on the glutamate-monoamine interactions in limbic lobe structures and their relevance for some psychiatric disorders

Pierre Magistretti

Dopaminergic, serotonergic and noradrenergic nuclei form the trimonoamine modulating system (TMMS). This system modulates emotional/motivational activities mediated by the limbic circuitry, where glutamate is the major excitatory neurotransmitter. Two main ...

2002

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