Concept

Énolase

Publications associées (8)

Pyruvate cellular uptake and enzymatic conversion probed by dissolution DNP-NMR: the impact of overexpressed membrane transporters

Arnaud Comment, Paul Romeo Vasos, Riccardo Balzan

Pyruvate membrane crossing and its lactate dehydrogenase-mediated conversion to lactate in cells featuring different levels of expression of membrane monocarboxylate transporters (MCT4) were probed by dissolution dynamic nuclear polarization-enhanced NMR. ...

Wiley2017

Role of the Glyoxalase System in Astrocyte-Mediated Neuroprotection

Pierre Magistretti, Igor Allaman, Thierry Laroche, Jean-Marie Petit, Jiangyan Yang

The glyoxalase system is the most important pathway for the detoxification of methylglyoxal (MG), a highly reactive dicarbonyl compound mainly formed as a by-product of glycolysis. MG is a major precursor of advanced glycation end products (AGEs), which ar ...

2011

Influence of residual ethanol concentration on the growth of Gluconacetobacter xylinus I 2281

Urs von Stockar, Ian William Marison, Henri Kornmann

The influence of residual ethanol on metab. of food grade Gluconacetobacter xylinum I 2281 was investigated during controlled cultivations on 35 g/l glucose and 5 g/l ethanol. Bacterial growth was strongly reduced in the presence of ethanol, which is unusu ...

Springer-Verlag2003

Quantitative analysis of the oxidative metabolism in HXK2- and REG1-deletion mutants of Saccharomyces cerevisiae

Urs von Stockar, Christoph Herwig

Deletion mutants with either deletion of HXK2 and REG1 of the wild-type strain Saccharomyces cerevisiae CEN.PK113-7D were quant. analyzed for the impact on carbon catabolite repression and the short-term effect of the oxidative metab. Therefore, continuous ...

2002

Noninvasive measurements of [1-13C]glycogen concentrations and metabolism in rat brain in vivo

Rolf Gruetter

Using a specific 13C NMR localization method, 13C label incorporation into the glycogen C1 resonance was measured while infusing [1- 13C]glucose in intact rats. The maximal concentration of [1-13C]glycogen was 5.1 ± 0.6 μmol g-1 (mean ± SE, n = 8). During ...

1999

Validation of 13C NMR measurements of liver glycogen in vivo

Rolf Gruetter

The natural abundance 13C NMR intensity of the glycogen C1 resonance was measured in the surgically exposed liver of rabbits in vivo (n = 17) by integration from 98 to 104 ppm and compared double blindedly to the subsequent biochemical measurement. Coil lo ...

1994

13C NMR visibility of rabbit muscle glycogen in vivo

Rolf Gruetter

The integrated 13C NMR intensity of the glycogen C1 resonance was measured in skeletal muscle (biceps femoris region) of nine rabbits under in vivo conditions. Concurrent chemical determinations of glycogen content showed that the in vivo signal was 1.02 ± ...

1991

Adenosine stimulates glycogenolysis in mouse cerebral cortex: a possible coupling mechanism between neuronal activity and energy metabolism

Pierre Magistretti, Jean-Luc Martin

Adenosine promotes a concentration-dependent hydrolysis of 3H-glycogen newly synthesized from 3H-glucose by mouse cerebral cortical slices. The EC50 for this effect is 7 microM. Theophylline antagonizes the glycogenolysis induced by adenosine with an EC50 ...

1986

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