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A review and discussion. Metab. is one of the most complex cellular processes. Connections between biochem. reactions via substrate and product metabolites create complex metabolic networks that may be analyzed using network theory, stoichiometric anal., a ...
A computational framework has been developed for the construction and evaluation of metabolic pathways given input substrates and knowledge of enzyme-catalyzed reactions. Application of the framework creates new and existing routes to both chems. known to ...
One of the central problems in metabolic engineering deals with the identification of the "rate limiting steps", i.e. enzymes whose activities one should manipulate towards the achievement of a desirable performance. However, uncertainty about the kinetic ...
A multitude of metabolic regulations occur in yeast, particularly under dynamic process conditions, such as under sudden glucose excess. However, quantification of regulations and classification of yeast strains under these conditions have yet to be elucid ...
The understanding of dynamic metabolic regulations is important for physiol. studies and strain characterization tasks. The present study combined transient expts. with online metabolic flux anal. (MFA) in order to quantify metabolic regulations, namely ca ...
The infrared (IR) absorption of a biological system can potentially report on fundamentally important microchemical properties. For example, molecular IR profiles are known to change during increases in metabolic flux, protein phosphorylation, or proteolyt ...
The effective biotechnol. process development depends on the system for monitoring the activity of the culture during cultivation. The feasibility of monitoring the metabolic flux during the cultivation of Bacillus thuringiensis var. galleriae (Btg) in pil ...
Astaxanthin is primarily used as a food supplement in the aquaculture industry, which over the past twenty years has experienced tremendous growth. A complex synthetic process produces almost all of the astaxanthin sold, and consequently, the selling price ...
Despite unquestionable evidence that glucose is the major energy substrate for the brain, data collected over several decades with different approaches suggest that lactate may represent a supplementary metabolic substrate for neurons. Starting with the pi ...
Growth of Methanobacterium thermoautotrophicum, an anaerobic archaebacterium using methanogenesis as the catabolic pathway, is characterized by large heat prodn. rates, up to 13 W g-1, and low biomass yields, in the order of 0.02 C-mol mol-1 H2 consumed. T ...