Concept

Scramblase

Publications associées (52)

Incorporation of Rhodopsin in Laterally Structured Supported Membranes: Observation of Transducin Activation with Spatially and Time-Resolved Surface Plasmon Resonance

Rhodopsin-transducin coupling was used as an assay to investigate a laterally patterned membrane reconstituted with a receptor and its G protein. It served as a model system to show the feasibility to immobilize G protein-coupled receptors on solid support ...

1998

Determination of the surface concentration of crown ethers in supported lipid membranes by capacitance measurements

Michael Graetzel, Horst Vogel, Samuel Terrettaz

The attachment of a tetradecyl chain to the ionophore 18-crown-6 leads to a stronger interaction with a supported lipid monolayer. Some C14-18-crown-6 mols. introduced in the aq. phase are incorporated in the phospholipid membrane. This process is used to ...

1998

A pore-forming toxin interacts with a GPI-anchored protein and causes vacuolation of the endoplasmic reticulum

Françoise Gisou van der Goot Grunberg, Laurence Gouzi Abrami

In this paper, we have investigated the effects of the pore-forming toxin aerolysin, produced by Aeromonas hydrophila, on mammalian cells. Our data indicate that the protoxin binds to an 80-kD glycosyl-phosphatidylinositol (GPI)-anchored protein on BHK cel ...

1998

Antibody Binding to a Functionalized Supported Lipid Layer: A Direct Acoustic Immunosensor

Horst Vogel, Martha Liley

A direct immunosensor has been developed using an acoustic wave device as a transducer. The device is based on an acoustic waveguide geometry that supports a Love wave. The biorecognition surface, formed on a gold layer, consisted of a biotinylated support ...

1997

Covalent attachment of functionalized lipid bilayers to planar waveguides for measuring protein binding to biomimetic membranes

Horst Vogel

A new method is presented for measuring sensitively the interactions between ligands and their membrane-bound receptors in situ by using integrated optics, thus avoiding the need for addnl. labels. Phospholipid bilayers were attached covalently to waveguid ...

1995

Biologically addressable monolayer structures formed by templates of sulfur-bearing molecules

Horst Vogel, Martha Liley, Claus Duschl

We demonstrate that the combined application of Langmuir-Blodgett and self-assembly techniques allows the fabrication of patterns with contrasting surface properties on gold substrates. The process is monitored using fluoroscence microscopy and surface pla ...

1994

Phospholipid asymmetry of the outer membrane of rat liver mitochondria: Evidence for the presence of cardiolipin on the outside of the outer membrane

Rudolf Hovius

The phospholipid topology of the outer membrane of intact rat liver mitochondria and derived outer membrane vesicles was investigated by determining the accessible pool of the various phospholipid classes towards phospholipase A2, a phosphatidylcholine-spe ...

Elsevier1993

Role of acidic lipids in the translocation and channel activity of colicins A and N in Escherichia coli cells

Françoise Gisou van der Goot Grunberg

Colicins A and N are pore-forming bacterial toxins that kill Escherichia coli cells. Their mode of action involves three steps; binding to specific receptors located in the outer membrane, translocation through this membrane and the periplasm, and channel ...

1993

On the mechanism of the mitochondrial decarboxylation of phosphatidylserine

Rudolf Hovius

To study intramitochondrial phospholipid flow, radiolabeled phosphatidylserine was introduced into isolated rat liver mitochondria from donor vesicles through the action of a nonspecific lipid transfer protein. Imported phosphatidylserine was rapidly decar ...

American Society for Biochemistry and Molecular Biology1992

Interaction of mitochondrial creatine kinase with model membranes: A monolayer study

Rudolf Hovius

The interaction of mitochondrial creatine kinase (Mi-CK; EC 2.7.3.2) with phospholipid monolayers and spread mitochondrial membranes at the air/water interface has been investigated. It appeared that Mi-CK penetrated into these monolayers as evidenced by a ...

Elsevier1991

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