Intracellular transport

Résumé

Intracellular transport is the movement of vesicles and substances within a cell. Intracellular transport is required for maintaining homeostasis within the cell by responding to physiological signals. Proteins synthesized in the cytosol are distributed to their respective organelles, according to their specific amino acid’s sorting sequence. Eukaryotic cells transport packets of components to particular intracellular locations by attaching them to molecular motors that haul them along microtubules and actin filaments. Since intracellular transport heavily relies on microtubules for movement, the components of the cytoskeleton play a vital role in trafficking vesicles between organelles and the plasma membrane by providing mechanical support. Through this pathway, it is possible to facilitate the movement of essential molecules such as membrane‐bounded vesicles and organelles, mRNA, and chromosomes. Intracellular transport is unique to eukaryotic cells because they possess organelles

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https://en.wikipedia.org/wiki/Intracellular_transport

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Monitoring the Cellular Cholesterol Distribution via NanoSIMS Imaging

Mustafa Demir

Cholesterol is a very crucial lipid for animal cells, since it is the building block of cellular membranes, control fluidity of membranes, regulate intracellular transport, cell signalling, nerve conduction and serve as a precursor for synthesis of vitamin D and steroid hormones. Cholesterol is such an important biological molecule for animal cells, yet it has been a real challenge so far, to understand distribution of cholesterol within the biological membranes. In our study, using ultra-high resolution ion microprobe (NanoSIMS) imaging enables us to detect incorporated cholesterol in cells, introduced as 13C labeled cholesterol, in nanometer scale. Then, we are able to monitor the localization of detected cholesterol in cellular structures by combining NanoSIMS (Secondary Ion Mass Spectrometry) with electron microscopy. Our aim in this study is to determine level of incorporated cholesterol in cellular membranes, secretion and endocytic systems, by the help of NanoSIMS imaging combined with electron microscopy.

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The molecular targeted drug ATRA demands a suitable carrier that delivers to the cancer site due to its poor bioavailability and drug resistance. ATRA, being a lipid with carboxylic acid, has been nano-formulated as a cationic lipo-ATRA with DOTAP:cholesterol:ATRA (5:4:1) and its pH-responsive release, intracellular drug accumulation, and anticancer effect on human lung cancer (A549) cell line analysed. The analysis of the physicochemical characteristics of the developed lipo-ATRA (0.8 mu mol) revealed that the size of 231 +/- 2.35 d.nm had a zeta potential of 6.4 +/- 1.19 and an encapsulation efficiency of 93.7 +/- 3.6%. The ATRA release from lipo-ATRA in vitro was significantly (p

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