Concept

Cytométrie en flux

Publications associées (221)

Capillary Microfluidic Chips for Point-of-Care Testing

Research on microfluidic devices for biological analysis has progressed sufficiently to be developed into point-of-care diagnostics products. The goal of this thesis is to improve multiple aspects of capillary-driven microfluidic devices. In particular, th ...

EPFL2011

Semisynthesis of fluorescent metabolite sensors on cell surfaces

Horst Vogel, Kai Johnsson, Luc Reymond, Rudolf Griss, Joachim Piguet, Matthias Albert Brun, Kui Thong Tan, Ruud Peters

Progress in understanding signal transduction and metabolic pathways is hampered by a shortage of suitable sensors for tracking metabolites, second messengers, and neurotransmitters in living cells. Here we introduce a class of rationally designed semisynt ...

2011

Relaxation Dynamics of Tryptophan in Water: A UV Fluorescence Up-Conversion and Molecular Dynamics Study

Majed Chergui, Frank van Mourik, Andrea Cannizzo, Ahmad Ajdar Zadeh Oskouei

We report on an ultrafast experimental and simulations study of the early relaxation events of photoexcited tryptophan in water. Experimentally, we used fluorescence up-conversion in both polychromatic and single wavelength detection modes in the 300-480 n ...

American Chemical Society2010

Cell Morphology and Intracellular Ionic Homeostasis explored with a Multimodal Approach combining Epifluorescence and Digital Holographic Microscopy

Pierre Magistretti, Christian Depeursinge, Nicolas Pavillon, Pierre Marquet, Jonas Kühn, Pascal Jourdain, Daniel Boss, Aleksandr Benke, Corinne Moratal

The authors have developed a live-cell multimodality microscope combining epifluorescence with digital holographic microscopy; it has been implemented with a decoupling procedure allowing to separately measure from the quantitative phase important cell par ...

2010

Fc Block Treatment, Dead Cells Exclusion, and Cell Aggregates Discrimination Concur to Prevent Phenotypical Artifacts in the Analysis of Subpopulations of Tumor-Infiltrating CD11b(+) Myelomonocytic Cells

It is well established that cancer cells can recruit CD11b(+) myeloid cells to promote tumor angiogenesis and tumor growth. Increasing interest has emerged on the identification of subpopulations of tumor-infiltrating CD11b(+) myeloid cells using flow cyto ...

2010

Ultrafast Excited-State Dynamics ef [Re(L)(CO)(3)(bpy)](n) Complexes: Involvement of the Solvent

Majed Chergui, Frank van Mourik, Andrea Cannizzo, Amal El Nahhas

Ultrafast excited-state dynamics of Re(L)(CO)(3)(bpy) (L = Cl, Br, n = 0; L = 4-ethyl-pyridine (Etpy), n = 1+; bpy = 2,2'-bipyridine) have been investigated in dimethylformamide (DMF) solution by fluorescence up-conversion (FIUC) and UV - vis transien ...

American Chemical Society2010

A miniaturized continuous dielectrophoretic cell sorter and its applications

Philippe Renaud, Thomas Michael Braschler, Nicolas Demierre, Ana Valero

There is great interest in highly sensitive separation methods capable of quickly isolating a particular cell type within a single manipulation step prior to their analysis. We present a cell sorting device based on the opposition of dielectrophoretic forc ...

American Institute of Physics2010

Microelectronics Meets Biology

Emile Dupont

Microfluidics plays a key role in the design of automated platforms for realizing biological assays in a miniaturized format. Several advantages are offered by a microfluidic system, namely, low sample and reagent consumption (typically a few microliters), ...

EPFL2010

Biomolecular Diffusion in Nanofluidics

Nicolas Durand

Imagine a small integrated biomedical analysis laboratory, connected to your home computer, which would be capable of diagnosing illnesses, a lack of vitamins, or the over-presence of substances from samples of blood, urine or saliva. This hypothetic syste ...

EPFL2010

Evidence for Drug Release from a Metalla-Cage Delivery Vector Following Cellular Internalisation

Paul Joseph Dyson, Olivier Pierre Zava

The authors have encapsulated in the cavity of [Ru6(p-iPrC6H4Me)6(tpt)2-(C6H2O4)3]6+ (tpt = 2,4,6-tris(pyridin-4-yl)-1,3,5-triazine), and intrinsically fluorescent pyrenyl compd. (pyrene-R = 1-(4,6-dichloro-1,3,5-trianzin-2-yl)pyrene), thus giving rise to ...

2010

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