Concept
Étanercept
Publications associées (7)
Human PBMCs Form Lipid Droplets in Response to Spike Proteins
Florian Maria Wurm, Guillaume Raussin
Intracellular lipid droplets (LDs) can accumulate in response to inflammation, metabolic stresses, and other physiological/pathological processes. Herein, we investigated whether spike proteins of SARS-CoV-2 induce LDs in human peripheral blood mononuclear ...
Basel2023Efficient and reproducible mammalian cell bioprocesses without probes and controllers?
Florian Maria Wurm, Mohamed Farhat, David Hacker, Lucia Baldi Unser, Matthieu Dreyer, Stéphanie Tissot, Martino Reclari, Agata Oberbek
Bioprocesses for recombinant protein production with mammalian cells are typically controlled for several physicochemical parameters including the pH and dissolved oxygen concentration (DO) of the culture medium. Here we studied whether these controls are ...
Elsevier2011Lentiviral Vector-Mediated Gene Delivery for the Generation of Chinese Hamster Ovary Cell Lines with Improved Productivity and Stability
Recombinant cell line generation by standard transfection techniques is a time-consuming and labor-intensive process often leading to an unpredictable outcome as transgene integration is a rare, random event. Consequently, the population of cells obtained ...
EPFL2010Immunoprivileged status of the liver is controlled by Toll-like receptor 3 signaling
The liver is known to be a classical immunoprivileged site with a relatively high resistance against immune responses. Here we demonstrate that highly activated liver-specific effector CD8+ T cells alone were not sufficient to trigger immune destruction of ...
2006Influence of cell-line and process conditions on the glycosylation of recombinant proteins
The final glycosylation pattern of recombinant proteins might be determined by the choice of the host cell, cell culture conditions and the purification. Each step influences glycosylation and improved glycosylation of the product may thus be obtained by c ...
EPFL2002Transduction of CD34+ cells with lentiviral vectors enables the production of large quantities of transgene-expressing immature and mature dendritic cells
BACKGROUND: Genetically engineered dendritic cells (DC) presenting specific antigens to T cells may be of great interest for immunotherapy. For this reason, the production of transgene-expressing DC derived from CD34 + cells transduced either shortly after ...
2001Mice that lack the interferon-gamma receptor have profoundly altered responses to infection with Bacillus Calmette-Guerin and subsequent challenge with lipopolysaccharide
Mice with a targeted disruption of the interferon gamma receptor gene (IFN-gamma R0/0 mice) and control wild-type mice were inoculated with the Bacillus Calmette-Guerin (BCG) strain of Mycobacterium bovis. BCG infection was not lethal for wild-type mice wh ...
1993