Concept

Microscope à statif inversé

Publications associées (60)

Resolution and Complex Deconvolution in Holographic Microscopy

Interest in high resolution imaging techniques has recently multiplied due to their importance in bio-medical research. Quantitative phase measurements by holographic microscopy is an extraordinary tool to gain new understanding of transparent biological s ...

EPFL2012

Quantitative Comparison of Commercial CCD and Custom-Designed CMOS Camera for Biological Applications

Giovanni De Micheli, Sandro Carrara, René Beuchat, Yusuf Leblebici, Gözen Köklü, Julien Michel Ghaye

In biological applications and systems where even the smallest details have a meaning, CCD cameras are mostly preferred and they hold most of the market share despite their high costs. In this paper, we propose a custom-designed CMOS camera to compete with ...

2012

Dynamic measurement of the height and volume of migrating cells by a novel fluorescence microscopy technique

Jean-Jacques Meister, Chiara Gabella, Lara Buscemi Estefanell, Benoît Vianay

We propose a new technique to measure the volume of adherent migrating cells. The method is based on a negative staining where a fluorescent, non-cell-permeant dye is added to the extracellular medium. The specimen is observed with a conventional fluoresce ...

2011

Angle-and polarization-dependent collective excitation of plasmonic nanoarrays for surface enhanced infrared spectroscopy

Hatice Altug, Shyamsunder Erramilli, Kai Chen

Our recent work has showed that diffractively coupled nanoplasmonic arrays for Fourier transform infrared (FTIR) microspectroscopy can enhance the Amide I protein vibrational stretch by up to 10(5) times as compared to plain substrates. In this work we con ...

2011

Experimental mapping of the spatial and angular emission patterns in photonic crystal microcavities

Laurent Balet, Massimo Gurioli

We report on a set of experimental measurements aimed to fully characterize both the spatial and angular pattern of the optical modes of photonic crystal microcavities. By using a confocal microscope we resolve the spatial distribution of the mode on the s ...

2010

Second harmonic generation from nanocrystals under linearly and circularly polarized excitations

Demetri Psaltis, Ye Pu, Rachel Grange, Chia-Lung Hsieh

We study second harmonic generation (SHG) from noncentrosymmetric nanocrystals under linearly polarized (LP) and circularly polarized (CP) excitations. Theoretical models are developed for SHG from nanocrystals under both plane-wave and focused excitations ...

2010

Extended depth-of-focus by digital holographic microscopy

Christian Depeursinge, Tristan Colomb, Etienne Cuche, Nicolas Pavillon, Jonas Kühn

A recurrent problem in microscopy is the finite depth-of-focus linked to the NA of microscope objectives. Digital holographic microscopy (DHM) has the unique feature of being able to numerically change the focus from a single hologram without the need of m ...

Optical Society of America2010

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Graphene has been dominating the electronic research community recently, with a brisk surge in proposals for its use in novel devices. The aspirations of 2D-carbon-based electronics largely rely on the availability of a mass-production technique to obtain ...

2010

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Vertical arrays of sealed nanofluidic channels, in which both cross-sectional dimensions are controllable down to 10 nm, were fabricated by selective side etching of a SiGe heterostructure comprised of layers of alternating Ge fractions. Capillary filling ...

2009

Digital holographic microscopy investigation of second harmonic generated at a glass/air interface

Christian Depeursinge, Nicolas Pavillon, Jonas Kühn, Etienne Shaffer

Optical second harmonic generation, thanks to its coherent nature, is a suitable signal for interferometric measurements, such as digital holography: a well-established imaging technique that allows recovering complex diffraction wavefields from which it i ...

2009

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