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Concept
Microsome
Résumé
In cell biology, microsomes are heterogeneous vesicle-like artifacts (~20-200 nm diameter) re-formed from pieces of the endoplasmic reticulum (ER) when eukaryotic cells are broken-up in the laboratory; microsomes are not present in healthy, living cells.
Rough (containing ribosomes) and smooth (without ribosomes) microsomes are made from the endoplasmic reticulum through cell disruption. These microsomes have an inside that is exactly the same as the endoplasmic reticulum lumen. Both forms of microsomes can be purified by a process known as equilibrium density centrifugation. Rough and smooth microsomes do differ in their proteins and rough microsomes have shown occurrence of translation and translocation at the same time besides certain exceptions from proteins in yeast.
The Signal Hypothesis was postulated by Günter Blobel and David Sabatini in 1971, stating that a unique peptide sequence is encoded by mRNA specific for proteins destined for translocation across the ER membrane. This peptide signal directs the active ribosome to the membrane surface and creates the conditions for transfer of the nascent polypeptide across the membrane. The generalization of the Signal Hypothesis to include signals for every organelle and location within the cell had an impact far beyond illuminating the targeting of secretory proteins, as it introduced the concept of 'topogenic' signals for the first time. Before the Signal Hypothesis, it was almost inconceivable that information encoded in the polypeptide chain could determine the localization of proteins in the cell.
This relates to cell-free protein synthesis. Cell-free protein synthesis that is without microsomes has no way for incorporation into the microsomes to happen. This means that when microsomal membranes are presented later there isn’t the removal of the signal sequence. With microsomes there, cell-free protein synthesis demonstrates cotranslational transport of the protein into the microsome and therefore the removal of the signal sequence. This process produces a mature protein chain.
Source officielle
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