Publications de Christian Depeursinge

Simultaneous dual-contrast three-dimensional imaging in live cells via optical diffraction tomography and fluorescence

Christian Depeursinge, Pierre Marquet, Chen Liu, Shanshan Kou

We report a dual-contrast method of simultaneously measuring and visualizing the volumetric structural information in live biological samples in three-dimensional (3D) space. By introducing a direct way of deriving the 3D scattering potential of the object ...

2019

Quantitative phase imaging in biomedicine

Christian Depeursinge

Quantitative phase imaging (QPI) has emerged as a valuable method for investigating cells and tissues. QPI operates on unlabelled specimens and, as such, is complementary to established fluorescence microscopy, exhibiting lower phototoxicity and no photobl ...

Nature Publishing Group2018

Quantitative phase-digital holographic microscopy: a new imaging modality to identify original cellular biomarkers of diseases

Pierre Magistretti, Christian Depeursinge, Benjamin Rappaz, Pierre Marquet, Pascal Jourdain, Kaspar Rothenfusser

Quantitative phase microscopy (QPM) has recently emerged as a powerful label-free technique in the field of living cell imaging allowing to non-invasively measure with a nanometric axial sensitivity cell structure and dynamics. Since the phase retardation ...

Spie-Int Soc Optical Engineering2016

Towards an incoherent off-axis digital holographic microscope

Christian Depeursinge, Frédéric Montfort

We propose and experimentally demonstrate a system in which off-axis digital holographic microscopy is realized using a broadband illumination source. Single-shot holographic measurements are enabled, while the coherence noise is removed thanks to the broa ...

Spie-Int Soc Optical Engineering2015

Off-axis digital holographic camera for quantitative phase microscopy

Christian Depeursinge, Christophe Moser, Frédéric Montfort, Zahra Monemhaghdoust

We propose and experimentally demonstrate a digital holographic camera which can be attached to the camera port of a conventional microscope for obtaining digital holograms in a self-reference configuration, under short coherence illumination and in a sing ...

Optical Soc Amer2014

Portable advanced off-axis camera for quantitative phase microscopy

Christian Depeursinge

We propose and experimentally demonstrate a device in which common-path interferometry combined with off-axis holographic geometry is used to realize a digital holographic camera which can be attached to the camera port of a conventional transmission micro ...

Spie-Int Soc Optical Engineering2014

Full field vertical scanning in short coherence digital holographic microscope

Christian Depeursinge, Christophe Moser, Etienne Cuche, Frédéric Montfort

In Digital holography Microscopes (DHM) implemented in the so-called "off axis" configuration, the object and reference wave fronts are not co-planar but form an angle of a few degrees. This results into two main drawbacks. First, the contrast of the inter ...

Optical Soc Amer2013

Quantitative phase noise in a two-color low coherence Digital Holographic Microscope

Christian Depeursinge, Christophe Moser, Zahra Monemhaghdoust, Frédéric Montfort

In digital holographic microscopy (DHM), the long coherence length of laser light causes parasitic interferences due to multiple reflections in and by optical components in the optical path of the microscope and thus degrades the image quality. The parasit ...

Spie-Int Soc Optical Engineering2013

A Practical Inverse-Problem Approach to Digital Holographic Reconstruction

Michaël Unser, Christian Depeursinge, Nicolas Pavillon, Emrah Bostan, Aurélien Bourquard

In this paper, we propose a new technique for high-quality reconstruction from single digital holographic acquisitions. The unknown complex object field is found as the solution of a nonlinear inverse problem that consists in the minimization of an energy ...

OSA2013

Apparatus and method for quantitative phase tomography through linear scanning with coherent and non-coherent detection

Christian Depeursinge, Nicolas Pavillon

The disclosed invention describes a new apparatus performing a new data acquisition for quantitative refractive index tomography. It is based on a linear scanning of the specimen, opposed to the classical approaches based on rotations of either the sample ...

2013