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Marcel Leutenegger

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Publications associées (67)

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Super-resolution opticalfluctuation imaging provides a resolution beyond the diffraction limitby analysing stochasticfluorescencefluctuations with higher-order statistics. Usingnthorderspatio-temporal cross-cumulants the spatial resolution and the sampling ...

2020

Combined Multi-Plane Tomographic Phase Retrieval and Stochastic Optical Fluctuation Imaging for 4D Cell Microscopy

Theo Lasser, Hilal Lashuel, Anne-Laure Mahul Mellier, Marcel Leutenegger, Emrah Bostan, Arno Pino Bouwens, Tomas Lukes, Adrien Charles-François Raymond Descloux

Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going beyond the diffraction barrier comes at a price since most far-field super-resolution imaging techniques trade temporal for spa ...

2018

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Super-resolution optical fluctuation imaging (SOFI) allows one to perform sub-diffraction fluorescence microscopy of living cells. By analyzing the acquired image sequence with an advanced correlation method, i.e. a high-order cross-cumulant analysis, supe ...

Public Library of Science2016

Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions

Theo Lasser, Aleksandra Radenovic, Lely Feletti, Marcel Leutenegger, Daniel Pawel Szlag, Tomas Lukes, Hendrik Georges Deschout, Azat Sharipov

Live-cell imaging of focal adhesions requires a sufficiently high temporal resolution, which remains a challenge for super-resolution microscopy. Here we address this important issue by combining photoactivated localization microscopy (PALM) with super-res ...

Nature Publishing Group2016

Stochastic optical fluctuation imaging (SOFI) is a super-resolution fluorescence imaging technique that makes use of stochastic fluctuations in the emission of the fluorophores. During a SOFI measurement multiple fluorescence images are acquired from the s ...

Optical Soc Amer2016

Optical prism for creating multiple views of an image

Theo Lasser, Marcel Leutenegger, Stefan Geissbuehler

Optical prism comprising two external parallel and reflecting surfaces, one internal interface, parallel to said surfaces and acting as light splitter; said prism furthermore comprising an entrance side forming an angle β with respect to said interface and ...

2016

Diffraction-unlimited fluorescence imaging allows the visualization of intact, strongly heterogeneous systems at unprecedented levels of detail. Beyond the acquisition of detailed pictures, increasing efforts are now being focused on deriving quantitative ...

Springer Verlag2015

Optical coherence correlation spectroscopy (OCCS)

Theo Lasser, Christophe Pache, Marcel Leutenegger, Arno Pino Bouwens, Stefan Geissbühler, Akihiro Sato, Stéphane Broillet

We present a new method called optical coherence correlation spectroscopy (OCCS) using nanoparticles as reporters of kinetic processes at the single particle level. OCCS is a spectral interferometry based method, thus giving simultaneous access to several ...

Optical Society of America2014

Visible light optical coherence correlation spectroscopy

Theo Lasser, Heinrich Hofmann, Marcel Leutenegger, Arno Pino Bouwens, Lionel André Maurizi, Daniel Pawel Szlag, Stéphane Broillet

Optical coherence correlation spectroscopy (OCCS) allows studying kinetic processes at the single particle level using the backscattered light of nanoparticles. We extend the possibilities of this technique by increasing its signal-to-noise ratio by a fact ...

Optical Society of America2014

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Super-resolution optical fluctuation imaging (SOFI) provides an elegant way of overcoming the diffraction limit in all three spatial dimensions by computing higher-order cumulants of image sequences of blinking fluorophores acquired with a classical widefi ...

2014