Publications de Michel Prudent

In vitro-transfusional model for red-blood-cell study: the advantage of lowering hematocrit

Gerardo Turcatti, Manon Sandra Bardyn, David Crettaz, Michel Prudent

Background - Thequalityof red blood cells (RBCs) stored in red cell concentrates (RCCs) is influenced by processing, storage and donor characteristics, and can have a clinical impact on transfused patients. To evaluate RBC properties and their potential im ...

Simtipro Srl2023

Image- and Fluorescence-Based Test Shows Oxidant-Dependent Damages in Red Blood Cells and Enables Screening of Potential Protective Molecules

Gerardo Turcatti, Benjamin Rappaz, David Crettaz, Michel Prudent, Manon Sandra Bardyn

An increase of oxygen saturation within blood bags and metabolic dysregulation occur during storage of red blood cells (RBCs). It leads to the gradual exhaustion of RBC antioxidant protective system and, consequently, to a deleterious state of oxidative st ...

2021

Quantification of stored red blood cell fluctuations by time-lapse holographic cell imaging

Gerardo Turcatti, Benjamin Rappaz, Pierre Marquet, Michel Prudent, Manon Sandra Bardyn

We propose methods to quantitatively calculate the fluctuation rate of red blood cells with nanometric axial and millisecond temporal sensitivity at the single-cell level by using time-lapse holographic cell imaging. For this quantitative analysis, cell me ...

2018

Immuno-affinity Amperometric Detection of Bacterial Infections

Hubert Girault, Horst Pick, Andreas Stephan Lesch, Michel Prudent, Milica Jovic, Yingdi Zhu

A combination of an immuno-affinity enrichment strategy and sensitive amperometric read-out was implemented in a point-of-care platform intended for bacterial infection analysis. Bacterial cells, selectively captured and enriched from complex matrices thro ...

Wiley-VCH Verlag GmbH2018

Red blood cells ageing markers: a multi-parametric analysis

Gerardo Turcatti, Niels Erik Lion, Benjamin Rappaz, David Crettaz, Michel Prudent, Manon Sandra Bardyn

Background. Red blood cells collected in citrate-phosphate-dextrose can be stored for up to 42 days at 4 degrees C in saline-adenine-glucose-mannitol additive solution. During this controlled, but nevertheless artificial, ex vivo ageing, red blood cells ac ...

Simti Servizi Srl2017

The antioxidant capacity of erythrocyte concentrates is increased during the first week of storage and correlated with the uric acid level

Hubert Girault, Niels Erik Lion, Andreas Stephan Lesch, Michel Prudent, Fernando Cortes Salazar, Sunny Isaïe Maye, Manon Sandra Bardyn

Background and Objectives: Red blood cells (RBCs) suffer from lesions during cold storage, depending in part on their ability to counterbalance oxidative stress by activating their antioxidant defence. The aim of this study was to monitor the antioxidant p ...

Wiley-Blackwell2017

Sensitive and fast identification of bacteria in blood samples by immunoaffinity mass spectrometry for quick BSI diagnosis

Hubert Girault, Horst Pick, Niels Erik Lion, Natalia Gasilova, Michel Prudent, Liang Qiao, Yingdi Zhu, Alexandra Bondarenko

Bloodstream infections rank among the most serious causes of morbidity and mortality in hospitalized patients, partly due to the long period (up to one week) required for clinical diagnosis. In this work, we have developed a sensitive method to quickly and ...

Royal Soc Chemistry2016

The Antioxidant Defense System of Erythrocyte Concentrates is Increased during the First Week of Storage

Michel Prudent, Niels Erik Lion, Fernando Cortes Salazar, Michael Müller

Wiley-Blackwell2016

Dynamic Metabolic Flux Analysis of Stored Red Blood Cells

Michel Prudent, Niels Erik Lion, Frédéric Stauber

Wiley-Blackwell2016

Molecular screening of cancer-derived exosomes by surface plasmon resonance spectroscopy

Horst Vogel, Niels Erik Lion, Davide Demurtas, Luigino Grasso, Romain Wyss, Michel Prudent, Ashwin Thampi, Lorenz Weidenauer

We report on a generic method to detect and iden- tify the molecular profile of exosomes either derived from cultured cell lines or isolated from biofluids. Exosomes are nanovesicles shed by cells into their microenvironment and carry the molecular identit ...

Springer Heidelberg2015