Lucia Baldi Unser

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Publications associées (55)

Transient Production of Recombinant Adeno-Associated Virus (AAV) Vectors for Gene Therapy Applications Using Suspension-Adapted HEK 293 Cells in Orbital Shaken Bioreactors

Florian Maria Wurm, Lucia Baldi Unser, Bernard Schneider, Daniel Roman Blessing, Monique Montavon, Nicole Déglon

Nature Publishing Group2015

Arginine-Based Biodegradable Ether–Ester Polymers with Low Cytotoxicity as Potential Gene Carriers

Lucia Baldi Unser, Jutta Christine Wandrey, Sagar Shashidhar Manoli, Nino Zavradashvili, Tamar Memanishvili

The success of gene therapy depends on safe and effective gene carriers. Despite being widely used, synthetic vectors based on poly(ethylenimine) (PEI), poly(l-lysine) (PLL), or poly(l-arginine) (poly-Arg) are not yet fully satisfactory. Thus, both improve ...

American Chemical Society2014

Virus-free transient protein production in Sf9 cells

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Xiao Shen

A method for virus-free transient gene expression from suspension-adapted Sf9 insect cells was developed with the gene of interest being expressed from a plasmid carrying the homologous region 5 enhancer (hr5) and the immediate early 1 (ie1) promoter from ...

Elsevier2014

Peptone Supplementation of Culture Medium Has Variable Effects on the Productivity of CHO Cells

Florian Maria Wurm, Lucia Baldi Unser, Yashas Rajendra, Fatemeh Davami

The optimization of cell culture conditions for growth and productivity of recombinant Chinese hamster ovary (CHO) cells is a critical step in biopharmaceutical manufacturing. In the present study, the effects of the timing and amount of peptone feeding of ...

2014

Cell Compatible Arginine Containing Cationic Polymer: One-Pot Synthesis and Preliminary Biological Assessment

Lucia Baldi Unser, Jutta Christine Wandrey, Xiao Shen, Nino Zavradashvili, Tamar Memanishvili

Synthetic cationic polymers are of interest as both nonviral vectors for intracellular gene delivery and antimicrobial agents. For both applications synthetic polymers containing guanidine groups are of special interest since such kind of organic compounds ...

Springer India2014

Disposable 600-mL orbitally shaken bioreactor for mammalian cell cultivation in suspension

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Dominique Terry Monteil Jr, Saroj Ghimire, Giulia Tontodonati

Orbitally shaken bioreactors (OSRs) support the efficient cultivation of mammalian cells in suspension at various scales of operation. Here we introduce a disposable culture vessel with a ventilated cap having a nominal volume of 600 mL (TubeSpin (R) biore ...

Elsevier2013

Helical-Track Bioreactors for Bacterial, Mammalian and Insect Cell Cultures

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Stéphanie Tissot, Clara Joséphine Douet

We investigated the cultivation of bacterial, mammalian and insect cells in an orbitally-shaken 250-mL disposable tube that incorporates a helical track (HT) on its inside wall. The mass transfer coefﬁcient of oxygen (kLa) was 200%–400% higher in the HT tu ...

2013

Study of the improved Sf9 transient gene expression process

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Xiao Shen

Insect cells have been widely used for the production of recombinant proteins using recombinant baculovirus for gene delivery [1]. To simplify protein production in insect cells, we have previously described a method, based on transient gene expression (TG ...

2013

Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Yashas Rajendra, Divor Kiseljak, Sarah Thurnheer

Transient gene expression (TGE) from mammalian cells is an increasingly important tool for the rapid production of recombinant proteins for research applications in biochemistry, structural biology, and biomedicine. Here we review methods for the transfect ...

Elsevier2013

The optimization of a rapid low-cost alternative of large-scale medium sterilization

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Dominique Terry Monteil Jr

2013

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Arginine-Based Biodegradable Ether–Ester Polymers with Low Cytotoxicity as Potential Gene Carriers

Lucia Baldi Unser, Jutta Christine Wandrey, Sagar Shashidhar Manoli, Nino Zavradashvili, Tamar Memanishvili

American Chemical Society2014

Cell Compatible Arginine Containing Cationic Polymer: One-Pot Synthesis and Preliminary Biological Assessment

Lucia Baldi Unser, Jutta Christine Wandrey, Xiao Shen, Nino Zavradashvili, Tamar Memanishvili

Springer India2014

Transient Production of Recombinant Adeno-Associated Virus (AAV) Vectors for Gene Therapy Applications Using Suspension-Adapted HEK 293 Cells in Orbital Shaken Bioreactors

Florian Maria Wurm, Lucia Baldi Unser, Bernard Schneider, Daniel Roman Blessing, Monique Montavon, Nicole Déglon

Nature Publishing Group2015