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Frequency-switched single-transition cross-polarization (FS-ST-CP) provides a versatile-tool for selective coherence transfer in heteronuclear NMR of biomols. such as proteins and nucleic acids. This type of coherence transfer is spin-state-selective and c ...
Cross-correlated fluctuations of isotropic chem. shifts can provide evidence for slow motions in biomols. Slow side-chain dynamics have been investigated in 15N and 13C enriched ubiquitin by monitoring the relaxation of Ca-Cb two-spin coherences (Frueh et ...
When applying multiple refocusing pulses to characterize the cross-correlated relaxation of heteronuclear multiple quantum coherence 2NxHx in biomols., the unavoidable effects of pulse imperfections are compensated by the scalar couplings between nitrogen ...
A novel NMR method characterizes slow motions in proteins by multiple refocusing of double- and zero-quantum coherences of amide protons and nitrogen-15 nuclei. If both nuclei experience changes in their isotropic chem. shifts because of internal motions o ...
In the present study the authors examine the thermodn. of binding of two related pyrazine-derived ligands to the major urinary protein, MUP-I, using a combination of isothermal titrn. calorimetry (ITC), x-ray crystallog., and NMR backbone 15N and Me side-c ...
The principal components and orientations of the chem. shift anisotropy (CSA) tensors of nearly all 13C carbonyl nuclei in a small protein have been detd. in isotropic soln. by a combination of three complementary cross-correlation measurements. [on SciFin ...
On page 2543, the bipolar scheme uses two gradient pulses for encoding, and two other gradient pulses for decoding. Thus, Equation 1 should be S/S0 = exp{-D4k2(D + 6t)}, rather than S/S0 = exp{-Dk2(D + 6t)}, as published. The incorrect expression would lea ...
In this paper some effects of dynamical coupling between two interaction-carrying vectors on the internal auto- and cross-correlation functions are investigated in the limit of small amplitude motions. A linearized Langevin approach allows the derivation o ...
A new expt. allows the identification of residues that feature slow conformational exchange in macromols. Rotations about dihedral angles that are slower than the global correlation time tc cause a modulation of the isotropic chem. shifts of the nuclei. If ...
A new NMR method has been designed for the measurement of the longitudinal relaxation rates of both donor and acceptor nitrogen-15 nuclei in Watson-Crick base pairs in 15N-enriched nucleic acids. The expt. was applied to a 22-nucleotide RNA hairpin. The le ...
