Publications de Caroline Vandevyver

Synthesis and cell localization of self-assembled dinuclear lanthanide bioprobes

Jean-Claude Bünzli, Anne-Sophie Chauvin, Caroline Vandevyver, Bo Song

Lanthanide bioprobes and bioconjugates are ideal luminescent stains in view of their low propensity to photobleaching, sharp emission lines and long excited state lifetimes permitting time-resolved detection for enhanced sensitivity. In this paper, we expa ...

Royal Society, The2013

Fluorescent magnetic bead and cell differentiation/counting using a CMOS SPAD matrix

Edoardo Charbon, Martinus Gijs, Ulrike Lehmann, Caroline Vandevyver, Emile Dupont

We present a monolithic silicon chip comprising a matrix of 84 single photon avalanche diodes (SPADs) to detect and discriminate fluorescent beads or fluorescently labeled single cells in a polydimethyl(-siloxane) (PDMS) cartridge that is positioned on top ...

Elsevier2012

Lighting-up cancerous cells and tissues with lanthanide luminescence

Martinus Gijs, Jean-Claude Bünzli, Anne-Sophie Chauvin, Caroline Vandevyver, Hans-Anton Lehr

2011

Chaotic mixing using source-sink microfluidic flows in a PDMS chip

Martinus Gijs, Abdeljalil Sayah, Caroline Vandevyver, Hüseyin Cumhur Tekin, Ata Tuna Ciftlik, Venkataragavalu Sivagnanam

We present an active fixed-volume mixer based on the creation of multiple source–sink microfluidic flows in a polydimethylsiloxane (PDMS) chip without the need of external or internal pumps. To do so, four different pressure-controlled actuation chambers a ...

Springer-Verlag2011

Fast Immunohistochemical BioMarker Detection Device for Cancer Tissue Slices

Martinus Gijs, Jean-Claude Bünzli, Caroline Vandevyver, Ata Tuna Ciftlik, Bo Song, Hans-Anton Lehr

2010

Active microfluidic mixer using virtual source-sink pairs for DNA purification

Martinus Gijs, Caroline Vandevyver, Hüseyin Cumhur Tekin

We present an active mixer based on the creation of virtual source-sink pairs in a polydimethylsiloxane (PDMS) chip. Perfect mixing is achieved in only a sub-second time interval for a compact 5 μL mixing volume. This excellent performance is achieved than ...

2010

Monolithic Silicon Chip for Immunofluorescence Detection on Single Magnetic Beads

Edoardo Charbon, Martinus Gijs, Ulrike Lehmann, Caroline Vandevyver, Emile Dupont

While fluorescence detection is widely used for bioassays owing to its high sensitivity, a complete fluorescent microscopy setup, comprised of a light source, optical filters, a microscope body, and a camera, still is bulky equipment, compromising its use ...

2010

Bioconjugated lanthanide luminescent helicates as multilabels for lab-on-a-chip detection of cancer biomarkers

Martinus Gijs, Jean-Claude Bünzli, Anne-Sophie Chauvin, Caroline Vandevyver, Venkataragavalu Sivagnanam, Bo Song, Vanesa Fernandez Moreira, Hans-Anton Lehr

The lanthanide binuclear helicate [Eu2(LC2(CO2H))3] is coupled to avidin to yield a luminescent bioconjugate EuB1 (Q ¼ 9.3%, s(5D0) ¼ 2.17 ms). MALDI/TOF mass spectrometry confirms the covalent binding of the Eu chelate and UV-visible spectroscopy allows o ...

2010

Sandwich immunoassay on a microfluidic chip using patterns of electrostatically self-assembled streptavidin-coated beads

Martinus Gijs, Caroline Vandevyver, Frédéric Lacharme, Venkataragavalu Sivagnanam

In this paper, we demonstrate a bead-based microfluidic immunoassay using self-assembled streptavidin-coated bead micropatterns. The beads are assembled on the microfluidic channel surface using electrostatic forces between the negatively-charged streptavi ...

Elsevier2009

Time-resolved lanthanide luminescence for lab-on-a-chip detection of biomarkers on cancerous tissues

Martinus Gijs, Jean-Claude Bünzli, Caroline Vandevyver, Venkataragavalu Sivagnanam, Bo Song, Hans-Anton Lehr

PDMS-based microfluidic devices combined with lanthanide-based immunocomplexes have been successfully tested for the multiplex detection of biomarkers on cancerous tissues, revealing an enhanced sensitivity compared to classical organic dyes. ...

2009