Publications de Philipp Wenter

Probing Mechanism and Transition State of RNA Refolding

Kinetics and the atomic detail of RNA refolding are only poorly understood. It has been proposed that conformations with transient base pairing interaction are populated during RNA refolding, but a detailed description of those states is lacking. By NMR an ...

2010

NMR-spectroscopic characterization of phosphodiester bond cleavage catalyzed by the minimal hammerhead ribozyme

Philipp Wenter

In order to relate the conformational dynamics of the hammerhead ribozyme to its biological function the cleavage reaction catalyzed by the hammerhead ribozyme was monitored by time-resolved nuclear magnetic resonance (NMR) spectroscopy. For this purpose, ...

2008

Conformational dynamics of bistable RNAs studied by time-resolved NMR spectroscopy

Luc Reymond, Philipp Wenter

The structural transition between two alternate conformations of bistable RNAs has been characterized by time-resolved NMR spectroscopy. The mechanism, kinetics, and thermodynamics underlying the global structural transition of bistable RNAs were delineate ...

2007

Kinetics of RNA Refolding in Dynamic Equilibrium by 1H-Detected 15N Exchange NMR Spectroscopy

Geoffrey Bodenhausen, Philipp Wenter

By implementing new NMR methods that were designed to map very slow exchange processes we have investigated and characterized the refolding kinetics of a thermodynamically stable 34mer RNA sequence in dynamic equilibrium. The RNA sequence was designed to u ...

2006

New methods for the investigation of RNA refolding by NMR spectroscopy

Philipp Wenter

A detailed characterization of the time scales and mechanisms by which RNA molecules change their folding structure upon binding of metabolites or during catalysis is important for the understanding of the different biological functions of RNA. The folding ...

EPFL2006

Short, synthetic and selectively 13C-labeled RNA sequences for the NMR structure determination of protein–RNA complexes

Luc Reymond, Philipp Wenter

We report an optimized synthesis of all canonical 2'-O-TOM protected ribonucleoside phosphoramidites and solid supports containing [13C5]-labeled ribose moieties, their sequence-specific introduction into very short RNA sequences and their use for the stru ...

Oxford University Press2006

Preparation of modified RNA sequences for biological research

Luc Reymond, Philipp Wenter, Sébastien Porcher, Frédéric David Meylan, Evelyne Muller, Damian Ackermann

Our group is working on the development of reliable access to biol. relevant, long, and modified RNA sequences. Here, we briefly present the chem. synthesis of such sequences with 2'-O-triisopropylsilyloxymethyl (= 2'-O-tom) protected ribonucleoside phosph ...

2005

Kinetics of photoinduced RNA refolding by real-time NMR spectroscopy

Philipp Wenter

By introducing a photolabile group in the Watson-Crick base-pairing site of a guanosine residue, a bistable 20-base RNA sequence was forced into a less stable conformation. A single laser pulse released the native sequence, and the subsequent refolding equ ...

Wiley-VCH Verlag GmbH2005

Structure of PTB Bound to RNA: Specific Binding and Implications for Splicing Regulation

Luc Reymond, Philipp Wenter

The polypyrimidine tract binding protein (PTB) is a 58-kDa RNA binding protein involved in multiple aspects of mRNA metab., including the repression of alternative exons. We have detd. the soln. structures of the four RNA binding domains (RBDs) of PTB, eac ...

American Association for the Advancement of Science2005