Our group is working on the development of reliable access to biol. relevant, long, and modified RNA sequences. Here, we briefly present the chem. synthesis of such sequences with 2'-O-triisopropylsilyloxymethyl (= 2'-O-tom) protected ribonucleoside phosph ...
The replication of Moloney murine leukaemia virus relies on the formation of a stable homodimeric 'kissing complex' of a GACG tetraloop interacting through only two C center dot G base pairs flanked of 5'-adjacent Unpaired adenosines A9. Previous NMR inves ...
More than 100 modified nucleosides have been found in naturally occurring RNA sequences. These modifications are involved in the correct folding, in the maintenance of the reading frame during translation and in the proper interaction with enzymes and prot ...
We report the synthesis of a modified 8-mer RNA sequence, (C-C-C-C-A-C-C-(2'-thio)A)-RNA 5'-(dihydrogen phosphate) contg. a 3'-terminal 2'-thio-adenosine and its spontaneous and site-specific aminoacylation with the weakly activated amino acid thioester H- ...
The first results of a study aiming at an efficient prepn. of a large variety of 2'-O-[(triisopropylsilyl)oxy]methyl(=tom)-protected ribonucleoside phosphoramidite building blocks contg. modified nucleobases are reported. All of the here presented nucleosi ...
Nucleic acid sequence analogues, in particular tRNA analogues comprising in the place of a 3'-terminal adenosine a 3'-terminal 2'-deoxy-2'-thioadenosine are able to react site-specifically with weakly activated amino acid derivatives (e.g. phenylthioesters ...
