Personne
Nicolas Bouche
Cette personne n’est plus à l’EPFL
Publications associées (13)
In vivo transcriptomic profiling using cell encapsulation identifies effector pathways of systemic aging
Patrick Descombes, Sylviane Métairon, Omid Mashinchian, Filippo De Franceschi, Nicolas Bouche, Christophe Boss
Sustained exposure to a young systemic environment rejuvenates aged organisms and promotes cellular function. However, due to the intrinsic complexity of tissues it remains challenging to pinpoint niche-independent effects of circulating factors on specifi ...
2022Pancreatic beta cell based optical biosensor and system for continuous glucose monitoring
Catherine Dehollain, Umberto De Marchi, Bakul Jitendra Vinchhi, Nicolas Bouche, Christophe Boss, Ninad Dilip Agashe
A bio-electronics system that measures cytosolic [Ca2+] in vitro in the INS-1E pancreatic beta-cells using a 430 nm excitation light with the excitation wavelength of the probe being at 435 nm was successfully implemented and tested. We transfected the INS ...
IEEE2019Optical pancreatic beta cell based biosensor, applications and glucose monitoring
Catherine Dehollain, Umberto De Marchi, Bakul Jitendra Vinchhi, Nicolas Bouche, Christophe Boss
An optical pancreatic beta-cell based biosensor for measuring cytosolic [Ca2+] is presented in this work. An in vitro bio-electronic system is built for testing various applications and characterizing features of the biosensor. A panoply of applications su ...
IEEE2019Encapsulation of Insulin-Secreting Cells Expressing a Genetically Encoded Fluorescent Calcium Indicator for Cell-Based Sensing In Vivo
Andreas Wiederkehr, Umberto De Marchi, Nicolas Bouche, Christophe Boss, Federico Sizzano, Mouna Conrad
The development of cell-based biosensors that give insight into cell and tissue function in vivo is an attractive technology for biomedical research. Here, the development of a cell line expressing a fluorescent calcium sensor for the study of beta-cell fu ...
Wiley2017Encapsulation device
Bernard Schneider, Nicolas Bouche, Aurélien Lathuilière
Provided herein is a cell encapsulation device comprising an internal chamber suitable for holding biological agents, wherein the internal chamber is disposed between a first semipermeable layer and a second semi-permeable layer, and the first and second s ...
2016System and Method For In Vivo Delivery of Antibodies and Fragments Thereof
Patrick Aebischer, Maurizio Molinari, Nicolas Bouche
The present invention is directed to devices and methods for in situ delivering an antibody or a fragment thereof to a host. In particular, the invention relates to devices and methods for in situ delivering an antibody or a fragment thereof to patient suf ...
2011Chronic delivery of antibody fragments using immunoisolated cell implants as a passive vaccination tool
Rolf Gruetter, Maria del Carmen Sandi Perez, Patrick Aebischer, Maurizio Molinari, Maria Isabel Cordero Campana, Jean-Charles Bensadoun, Nicolas Bouche, Veronica Setola, Osiris Marroquin Belaunzaran
BACKGROUND: Monoclonal antibodies and antibody fragments are powerful biotherapeutics for various debilitating diseases. However, high production costs, functional limitations such as inadequate pharmacokinetics and tissue accessibility are the current pri ...
Public Library of Science2011Cell encapsulation technology as a novel strategy for human anti-tumor immunotherapy
Vivianne Padrun, Nicolas Bouche
Granulocyte-macrophage colony-stimulating factor (GM-CSF) as an adjuvant in autologous cell-based anti-tumor immunotherapy has recently been approved for clinical application. To avoid the need for individualized processing of autologous cells, we develope ...
Nature Publishing Group2011An in vivo culture system for human embryos using an encapsulation technology: a pilot study
Patrick Aebischer, Nicolas Bouche
ACKGROUND: Animal studies have demonstrated better embryo development in vivo than in vitro. This pilot study tested the feasibility of using a novel in utero culture system (IUCS) to obtain normal human fertilization and embryo development. METHODS: The I ...
Oxford University Press2009In vivo calcium deposition on polyvinyl alcohol matrix used in hollow fiber cell macroencapsulation devices
Patrick Aebischer, Nicolas Bouche
The encapsulation of genetically modified cells represents a promising approach for the delivery of therapeutic proteins. The functionality of the device is dependent on the characteristics of the biomaterials, the procedures used in its confection and the ...
2004