Publications de Nicolas Bouche

In vivo transcriptomic profiling using cell encapsulation identifies effector pathways of systemic aging

Patrick Descombes, Jérome Feige, Sylviane Métairon, Omid Mashinchian, Filippo De Franceschi, Nicolas Bouche, Christophe Boss

Sustained exposure to a young systemic environment rejuvenates aged organisms and promotes cellular function. However, due to the intrinsic complexity of tissues it remains challenging to pinpoint niche-independent effects of circulating factors on specifi ...

2022

Pancreatic beta cell based optical biosensor and system for continuous glucose monitoring

Catherine Dehollain, Umberto De Marchi, Bakul Jitendra Vinchhi, Ninad Dilip Agashe, Nicolas Bouche, Christophe Boss

A bio-electronics system that measures cytosolic [Ca2+] in vitro in the INS-1E pancreatic beta-cells using a 430 nm excitation light with the excitation wavelength of the probe being at 435 nm was successfully implemented and tested. We transfected the INS ...

IEEE2019

Optical pancreatic beta cell based biosensor, applications and glucose monitoring

Catherine Dehollain, Umberto De Marchi, Bakul Jitendra Vinchhi, Nicolas Bouche, Christophe Boss

An optical pancreatic beta-cell based biosensor for measuring cytosolic [Ca2+] is presented in this work. An in vitro bio-electronic system is built for testing various applications and characterizing features of the biosensor. A panoply of applications su ...

IEEE2019

Encapsulation device

Bernard Schneider, Nicolas Bouche, Aurélien Lathuilière

2018

Encapsulation of Insulin-Secreting Cells Expressing a Genetically Encoded Fluorescent Calcium Indicator for Cell-Based Sensing In Vivo

Christophe Boss, Umberto De Marchi, Nicolas Bouche, Andreas Wiederkehr, Federico Sizzano, Mouna Conrad

The development of cell-based biosensors that give insight into cell and tissue function in vivo is an attractive technology for biomedical research. Here, the development of a cell line expressing a fluorescent calcium sensor for the study of beta-cell fu ...

Wiley2017

Encapsulation device

Bernard Schneider, Nicolas Bouche, Aurélien Lathuilière

Provided herein is a cell encapsulation device comprising an internal chamber suitable for holding biological agents, wherein the internal chamber is disposed between a first semipermeable layer and a second semi-permeable layer, and the first and second s ...

2016

5PDCell-based immunotherapy combining encapsulation cell technology and irradiated autologous tumor cells: A novel technology platform that is both safe and feasible. Results from first in man trial

Nicolas Bouche

Oxford, Oxford University Press2015

System and Method For In Vivo Delivery of Antibodies and Fragments Thereof

Patrick Aebischer, Maurizio Molinari, Nicolas Bouche

The present invention is directed to devices and methods for in situ delivering an antibody or a fragment thereof to a host. In particular, the invention relates to devices and methods for in situ delivering an antibody or a fragment thereof to patient suf ...

2011

Chronic delivery of antibody fragments using immunoisolated cell implants as a passive vaccination tool

Rolf Gruetter, Maria del Carmen Sandi Perez, Patrick Aebischer, Jean-Charles Bensadoun, Maurizio Molinari, Nicolas Bouche, Maria Isabel Cordero Campana, Veronica Setola, Osiris Marroquin Belaunzaran

BACKGROUND: Monoclonal antibodies and antibody fragments are powerful biotherapeutics for various debilitating diseases. However, high production costs, functional limitations such as inadequate pharmacokinetics and tissue accessibility are the current pri ...

Public Library of Science2011

An in vivo culture system for human embryos using an encapsulation technology: a pilot study

Patrick Aebischer, Nicolas Bouche

ACKGROUND: Animal studies have demonstrated better embryo development in vivo than in vitro. This pilot study tested the feasibility of using a novel in utero culture system (IUCS) to obtain normal human fertilization and embryo development. METHODS: The I ...

Oxford University Press2009