Publications de David Hacker | EPFL Graph Search

Changes in SARS-CoV-2 Spike versus Nucleoprotein Antibody Responses Impact the Estimates of Infections in Population-Based Seroprevalence Studies

Didier Trono, David Hacker, Florence Pojer, Kelvin Ka Ching Lau

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific antibody responses to the spike (S) protein monomer, S protein native trimeric form, or the nucleocapsid (N) proteins were evaluated in cohorts of individuals with acute infection (n = 9 ...

American Society for Microbiology2021

A Nanoscaffolded Spike-RBD Vaccine Provides Protection against SARS-CoV-2 with Minimal Anti-Scaffold Response

David Hacker, Bruno Emanuel Ferreira De Sousa Correia, Florence Pojer, Kelvin Ka Ching Lau

The response of the adaptive immune system is augmented by multimeric presentation of a specific antigen, resembling viral particles. Several vaccines have been designed based on natural or designed protein scaffolds, which exhibited a potent adaptive immu ...

2021

Analysis of volumetric mass transfer coefficient (k(L)a) in small- (250 mL) to large-scale (2500 L) orbitally shaken bioreactors

Florian Maria Wurm, David Hacker, Likuan Zhu

In this study, the combination of dimensional analysis (DA) and analysis of variance (ANOVA) was used to predict the volumetric mass transfer coefficient (k(L)a) values under different operating conditions for orbitally shaken bioreactors (OSRs) with diffe ...

SPRINGER HEIDELBERG2020

Structural investigation of ACE2 dependent disassembly of the trimeric SARS-CoV-2 Spike glycoprotein

Henning Paul-Julius Stahlberg, David Hacker, Florence Pojer, Kelvin Ka Ching Lau, Dongchun Ni

The human membrane protein Angiotensin-converting enzyme 2 (hACE2) acts as the main receptor for host cells invasion of the new coronavirus SARS-CoV-2. The viral surface glycoprotein Spike binds to hACE2, which triggers virus entry into cells. As of today, ...

2020

Chromatin three-dimensional interactions mediate genetic effects on gene expression

David Hacker, Giovanna Ambrosini, Philipp Bucher, Sanjeev Kumar

Studying the genetic basis of gene expression and chromatin organization is key to characterizing the effect of genetic variability on the function and structure of the human genome. Here we unravel how genetic variation perturbs gene regulation using a da ...

American Association for the Advancement of Science2019

BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing

Bart Deplancke, David Hacker, Bastien Mangeat, Vincent Roland Julien Gardeux, Julie Marie Russeil, Daniel Alpern, Antonio Carlos Alves Meireles Filho

Despite its widespread use, RNA-seq is still too laborious and expensive to replace RT-qPCR as the default gene expression analysis method. We present a novel approach, BRB-seq, which uses early multiplexing to produce 3 cDNA libraries for dozens of sample ...

2019

Chromatin 3D interactions mediate genetic effects on gene expression

David Hacker, Giovanna Ambrosini, Philipp Bucher

NATURE PUBLISHING GROUP2019

Improved process conditions for increasing expression of MHC class II protein from a stable Drosophila S2 cell line

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Xiao Shen

2018

Fluid dynamics of flow fields in a disposable 600-mL orbitally shaken bioreactor

Florian Maria Wurm, David Hacker, Dominique Terry Monteil Jr, Likuan Zhu

Orbitally shaken bioreactors (OSRs) are commonly used for the cultivation of mammalian cells in suspension. Here we conducted a three-dimensional computational fluid dynamics (CFD) simulation to characterize the fluid field in the disposable 600-mL orbital ...

Elsevier2018

Expression proteomics study to determine metallodrug targets and optimal drug combinations

Paul Joseph Dyson, Kai Johnsson, David Hacker, Claire Samantha Allardyce

The emerging technique termed functional identification of target by expression proteomics (FITExP) has been shown to identify the key protein targets of anti-cancer drugs. Here, we use this approach to elucidate the proteins involved in the mechanism of a ...

Nature Research2017