Publications de Stefan Geissbühler

Optical coherence correlation spectroscopy (OCCS)

Theo Lasser, Marcel Leutenegger, Christophe Pache, Arno Pino Bouwens, Stefan Geissbühler, Akihiro Sato, Stéphane Broillet

We present a new method called optical coherence correlation spectroscopy (OCCS) using nanoparticles as reporters of kinetic processes at the single particle level. OCCS is a spectral interferometry based method, thus giving simultaneous access to several ...

Optical Society of America2014

Structural and Functional Stochastic Super-Resolution Microscopy

Stefan Geissbühler

Super-resolution fluorescence microscopy is a promising tool with the potential to strengthen our understanding of living processes. Based on the ability to switch fluorophores on and off in an either deterministic or stochastic manner, the fundamental lim ...

EPFL2013

Comparison between SOFI and STORM

Theo Lasser, Stefan Geissbühler, Claudio Dellagiacoma

A straightforward method to achieve super-resolution consists of taking an image sequence of stochastically blinking emitters using a standard wide-field fluorescence microscope. Densely packed single molecules can be distinguished sequentially in time usi ...

2011

Targeted Photoswitchable Probe for Nanoscopy of Biological Structures

Theo Lasser, Kai Johnsson, Iwan Märki, Stefan Geissbühler, Noelia Laura Bocchio, Grazvydas Lukinavicius, Claudio Dellagiacoma, Sambashiva Banala

We introduce a photoswitchable O6-benzylguanine derivative and demonstrate its use for super-resolution microscopy of SNAP-tagged proteins based on single fluorophore localization. Stochastic Optical Reconstruction Microscopy (STORM) reveals SNAP-tagged mi ...

2010

Three-dimensional nano-localization of single fluorescent emitters

Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler, Noelia Laura Bocchio

We present a combination of self-interference microscopy with lateral super-resolution microscopy and introduce a novel approach for localizing a single nano-emitter to within a few nanometers in all three dimensions over a large axial range. We demonstrat ...

Optical Society of America2010

Super-resolved position and orientation estimation of fluorescent dipoles using 3-D steerable filters

Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler

The diffraction patterns of fixed fluorophores are characteristic of the orientation of the molecules' underlying dipole. Fluorescence localization microscopy techniques such as PALM and STORM achieve super-resolution by sequentially imaging sparse subsets ...

Spie-Int Soc Optical Engineering, Po Box 10, Bellingham, Wa 98227-0010 Usa2010

Steerable filters for orientation estimation and localization of fluorescent dipoles

Michaël Unser, Theo Lasser, Iwan Märki, Stefan Geissbühler, François Aguet

Fluorescence localization microscopy (i.e., PALM, STORM) has enabled optical imaging at nanometer-scale resolutions. The localization algorithms used in these techniques rely on fitting a 2-D Gaussian to the in-focus image of individual fluorophores. For f ...

Ieee Service Center, 445 Hoes Lane, Po Box 1331, Piscataway, Nj 08855-1331 Usa2009

Super-resolution orientation estimation and localization of fluorescent dipoles using 3-D steerable filters

Michaël Unser, Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler

Fluorophores that are fixed during image acquisition produce a diffraction pattern that is characteristic of the orientation of the fluorophore’s underlying dipole. Fluorescence localization microscopy techniques such as PALM and STORM achieve super-resolu ...

OSA2009