Publications de Christophe Pache

Three dimensional time lapse imaging of live cell mitochondria with photothermal optical lock-in optical coherence microscopy (Conference Presentation)

Theo Lasser, Christophe Pache, Miguel Sison, Jérôme Extermann, Amir Jean Nahas

Spie-Int Soc Optical Engineering2016

Combined Optical Coherence and Fluorescence Microscopy to assess dynamics and specificity of pancreatic beta-cell tracers

Theo Lasser, Anne Grapin-Botton, Arno Pino Bouwens, Christophe Pache, Daniel Pawel Szlag, Corinne Berclaz, Antonio Lopez

The identification of a beta-cell tracer is a major quest in diabetes research. However, since MRI, PET and SPECT cannot resolve individual islets, optical techniques are required to assess the specificity of these tracers. We propose to combine Optical Co ...

Nature Publishing Group2015

Optical coherence correlation spectroscopy (OCCS)

Theo Lasser, Marcel Leutenegger, Christophe Pache, Arno Pino Bouwens, Stefan Geissbühler, Akihiro Sato, Stéphane Broillet

We present a new method called optical coherence correlation spectroscopy (OCCS) using nanoparticles as reporters of kinetic processes at the single particle level. OCCS is a spectral interferometry based method, thus giving simultaneous access to several ...

Optical Society of America2014

Contrast Enhancement in Optical Coherence Microscopy

Christophe Pache

Optical Coherence Microscopy (OCM) is a three-dimensional imaging technique that provides cross-sectional views of the subsurface microstructure of biological tissue, with a high axial and lateral resolution. In OCM, a low time coherence light source is sp ...

EPFL2012

Fast three-dimensional imaging of gold nanoparticles in living cells with photothermal optical lock-in Optical Coherence Microscopy

Theo Lasser, Christian Santschi, Martin Villiger, Christophe Pache, Arno Pino Bouwens, Corinne Berclaz, Noelia Laura Bocchio, Matthew Gibson, Joan Goulley

We introduce photothermal optical lock-in Optical Coherence Microscopy (poli-OCM), a volumetric imaging technique, which combines the depth sectioning of OCM with the high sensitivity of photothermal microscopy while maintaining the fast acquisition speed ...

Optical Society of America2012

Label-free imaging of cerebral β-amyloidosis with extended-focus optical coherence microscopy

Theo Lasser, Patrick Fraering, Martin Villiger, Christophe Pache, Arno Pino Bouwens, Tristan Bolmont, Mitko Dimitrov, Corinne Berclaz

We demonstrate label-free imaging of cerebral β-amyloidosis ex vivo and in a living mouse model of Alzheimer's disease using extended focus Fourier domain optical coherence microscopy (xfOCM). xfOCM provides 3D, high-resolution images of individual β-amylo ...

Society for Neuroscience2012

Dark field optical coherence microscopy

Theo Lasser, Martin Villiger, Christophe Pache

The invention relates to an optical coherence microscopy system for fast, phase resolved imaging by means of optical coherence microscopy with decoupled illumination and detection apertures, producing a dark-field effect with an enhanced optical contrast. ...

2011

Dark-field optical coherence microscopy

Theo Lasser, Martin Villiger, Christophe Pache

Dark-field illumination is known to enhance scattering contrast in optical microscopy. We combined this concept with Fourier domain optical coherence microscopy (OCM). The detection and illumination paths are decoupled, and only the scattered light origina ...

Optical Society of America2010

Digital holographic microscopy real-time monitoring of cytoarchitectural alterations during simulated microgravity

Christian Depeursinge, Jonas Kühn, Muhammed Fatih Toy, Christophe Pache, Jérôme Parent, Marcel Egli

Previous investigations on mammalian cells have shown that microgravity, either that experienced in space, or simulated on earth, causes severe cellular modifications that compromise tissue determination and function. The aim of this study is to investigat ...

SPIE Society of Photo-optical Instrumentation Engineers2010