Publications de Joan Goulley | EPFL Graph Search

Longitudinal three-dimensional visualisation of autoimmune diabetes by functional optical coherence imaging

Theo Lasser, Martin Villiger, Anne Grapin-Botton, Arno Pino Bouwens, Corinne Berclaz, Daniel Pawel Szlag, Jérôme Extermann, Joan Goulley

Aims/hypothesis It is generally accepted that structural and functional quantitative imaging of individual islets would be beneficial to elucidate the pathogenesis of type 1 diabetes. We here introduce functional optical coherence imaging (FOCI) for fast, ...

Springer Verlag2016

Bicaudal C1 promotes pancreatic NEUROG3+ endocrine progenitor differentiation and ductal morphogenesis

Daniel Constam, Anne Grapin-Botton, Jacques Rougemont, Joan Goulley, Yung Hae Kim, Laurence Anne E Lemaire

In human, mutations in bicaudal C1 (BICC1), an RNA binding protein, have been identified in patients with kidney dysplasia. Deletion of Bicc1 in mouse leads to left-right asymmetry randomization and renal cysts. Here, we show that BICC1 is also expressed i ...

Company Of Biologists Ltd2015

Fast three-dimensional imaging of gold nanoparticles in living cells with photothermal optical lock-in Optical Coherence Microscopy

Theo Lasser, Christian Santschi, Martin Villiger, Christophe Pache, Arno Pino Bouwens, Corinne Berclaz, Noelia Laura Bocchio, Matthew Gibson, Joan Goulley

We introduce photothermal optical lock-in Optical Coherence Microscopy (poli-OCM), a volumetric imaging technique, which combines the depth sectioning of OCM with the high sensitivity of photothermal microscopy while maintaining the fast acquisition speed ...

Optical Society of America2012

Diabetes imaging — quantitative assessment of islets of Langerhans distribution in murine pancreas using extended-focus optical coherence microscopy

Dimitri Nestor Alice Van De Ville, Theo Lasser, Anthony Christopher Davison, Anne Grapin-Botton, Martin Villiger, Arno Pino Bouwens, Corinne Berclaz, Joan Goulley, Erica Martin-Williams

Diabetes is characterized by hyperglycemia that can result from the loss of pancreatic insulin secreting β-cells in the islets of Langerhans. We analyzed ex vivo the entire gastric and duodenal lobes of a murine pancreas using extended-focus Optical Cohere ...

2012

Towards high resolution optical imaging of beta cells in vivo

Theo Lasser, Martin Villiger, Anne Grapin-Botton, Erica Martin-Williams, Joan Goulley

Endocrine beta cells produce and release insulin in order to tightly regulate glucose homeostasis and prevent metabolic pathologies such as Diabetes Mellitus. Optical imaging has contributed greatly to our current understanding of beta cell structure and f ...

Bentham Science Publishers2010

In vivo imaging of murine endocrine islets of Langerhans with extended-focus optical coherence microscopy

Theo Lasser, Rainer Leitgeb, Anne Grapin-Botton, Martin Villiger, Joan Goulley, Michael Friedrich

Aims/hypothesis Structural and functional imaging of the islets of Langerhans and the insulin-secreting beta cells represents a significant challenge and a long-lasting objective in diabetes research. In vivo microscopy offers a valuable insight into beta ...

Springer Verlag2009