Publications de John McKinney | EPFL Graph Search

Investigating the composition and recruitment of the mycobacterial ImuA'-ImuB-DnaE2 mutasome

John McKinney, Neeraj Dhar, Zela Alexandria-Mae Martin

A DNA damage-inducible mutagenic gene cassette has been implicated in the emergence of drug resistance in Mycobacterium tuberculosis during anti-tuberculosis (TB) chemotherapy. However, the molecular composition and operation of the encoded 'mycobacterial ...

Cambridge2023

Preexisting Heterogeneity of Inducible Nitric Oxide Synthase Expression Drives Differential Growth of Mycobacterium tuberculosis in Macrophages

John McKinney, Ophélie Rutschmann, Chiara Toniolo

Mycobacterium tuberculosis infection is initiated by the inhalation and implantation of bacteria in the lung alveoli, where they are phagocytosed by macrophages. Even a single bacterium may be sufficient to initiate infection. Thereafter, the clinical outc ...

AMER SOC MICROBIOLOGY2022

Dynamic persistence of UPEC intracellular bacterial communities in a human bladder-chip model of urinary tract infection

John McKinney, Neeraj Dhar, Vivek Vijay Thacker, François Signorino-Gelo, Kunal Sharma, Thomas Marie Simonet

Uropathogenic Escherichia coli (UPEC) proliferate within superficial bladder umbrella cells to form intracellular bacterial communities (IBCs) during early stages of urinary tract infections. However, the dynamic responses of IBCs to host stresses and anti ...

2021

Revealing Antibiotic Tolerance of the Mycobacterium smegmatis Xanthine/Uracil Permease Mutant Using Microfluidics and Single-Cell Analysis

John McKinney, Neeraj Dhar, Meltem Elitas

To reveal rare phenotypes in bacterial populations, conventional microbiology tools should be advanced to generate rapid, quantitative, accurate, and high-throughput data. The main drawbacks of widely used traditional methods for antibiotic studies include ...

MDPI2021

Do chance encounters between heterogeneous cells shape the outcome of tuberculosis infections?

John McKinney, Ophélie Rutschmann, Chiara Toniolo

The sum of all of the interactions between single bacteria and host cells determines if an infection is cleared, controlled, or progresses at the whole host-organism level. These individual interactions have independent trajectories defined by diverse and ...

2020

A biphasic growth model for cell pole elongation in mycobacteria

Georg Fantner, John McKinney, Haig Alexander Eskandarian, Mélanie Thérèse Marie Hannebelle, Chiara Toniolo, Joëlle Xiao Yuan Ven, Gaëlle Madeleine Vuaridel

Mycobacteria grow by inserting new cell wall material in discrete zones at the cell poles. This pattern implies that polar growth zones must be assembled de novo at each division, but the mechanisms that control the initiation of new pole growth are unknow ...

2020

Overlapping and essential roles for molecular and mechanical mechanisms in mycobacterial cell division

Georg Fantner, John McKinney, Adrian Pascal Nievergelt, Pascal Damian Odermatt, Haig Alexander Eskandarian, Mélanie Thérèse Marie Hannebelle

Mechanisms to control cell division are essential for cell proliferation and survival. Bacterial cell growth and division require the coordinated activity of peptidoglycan synthases and hydrolytic enzymes to maintain mechanical integrity of the cell wall. ...

2019

Preexisting variation in DNA damage response predicts the fate of single mycobacteria under stress

John McKinney, Neeraj Dhar, Giulia Manina, Lalit Kumar Singh

Clonal microbial populations are inherently heterogeneous, and this diversification is often considered as an adaptation strategy. In clinical infections, phenotypic diversity is found to be associated with drug tolerance, which in turn could evolve into g ...

2019

Computational Analysis of the Mutual Constraints between Single‐Cell Growth and Division Control Models

John McKinney, Neeraj Dhar, Ambroise Roger Vuaridel

Three models of division control are proposed to achieve cell size homeostasis: sizer, timer, and adder. However, few published studies of division control take into account the dynamics of single‐cell growth and most assume that single‐cell growth is expo ...

2019

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John McKinney

Investigating the composition and recruitment of the mycobacterial ImuA'-ImuB-DnaE2 mutasome

Preexisting Heterogeneity of Inducible Nitric Oxide Synthase Expression Drives Differential Growth of Mycobacterium tuberculosis in Macrophages

Dynamic persistence of UPEC intracellular bacterial communities in a human bladder-chip model of urinary tract infection

Revealing Antibiotic Tolerance of the Mycobacterium smegmatis Xanthine/Uracil Permease Mutant Using Microfluidics and Single-Cell Analysis

Seeing and Touching the Mycomembrane at the Nanoscale

Do chance encounters between heterogeneous cells shape the outcome of tuberculosis infections?

A biphasic growth model for cell pole elongation in mycobacteria

Overlapping and essential roles for molecular and mechanical mechanisms in mycobacterial cell division

Preexisting variation in DNA damage response predicts the fate of single mycobacteria under stress

Computational Analysis of the Mutual Constraints between Single‐Cell Growth and Division Control Models

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Preexisting Heterogeneity of Inducible Nitric Oxide Synthase Expression Drives Differential Growth of Mycobacterium tuberculosis in Macrophages

Investigating the composition and recruitment of the mycobacterial ImuA'-ImuB-DnaE2 mutasome

Dynamic persistence of UPEC intracellular bacterial communities in a human bladder-chip model of urinary tract infection

Seeing and Touching the Mycomembrane at the Nanoscale

A biphasic growth model for cell pole elongation in mycobacteria

Overlapping and essential roles for molecular and mechanical mechanisms in mycobacterial cell division

Do chance encounters between heterogeneous cells shape the outcome of tuberculosis infections?

Computational Analysis of the Mutual Constraints between Single‐Cell Growth and Division Control Models

Preexisting variation in DNA damage response predicts the fate of single mycobacteria under stress

Revealing Antibiotic Tolerance of the Mycobacterium smegmatis Xanthine/Uracil Permease Mutant Using Microfluidics and Single-Cell Analysis