Publications de Menno Berend Tol

Structure of tetrameric forms of the serotonin-gated 5-HT3A receptor ion channel

Multimeric membrane proteins are produced in the endoplasmic reticulum and transported to their target membranes which, for ion channels, is typically the plasma membrane. Despite the availability of many fully assembled channel structures, our understandi ...

SPRINGERNATURE2024

X-ray structure of the mouse serotonin 5-HT3 receptor

Horst Vogel, Henning Paul-Julius Stahlberg, Rudolf Hovius, Cédric Deluz, Luigino Grasso, Romain Wyss, Menno Berend Tol, Ghérici Hassaïne

Neurotransmitter-gated ion channels of the Cys-loop receptor family mediate fast neurotransmission throughout the nervous system. The molecular processes of neurotransmitter binding, subsequent opening of the ion channel and ion permeation remain poorly un ...

Nature Publishing Group2014

Large scale expression and purification of the mouse 5-HT3 receptor (vol 1828, pg 2544, 2013)

Horst Vogel, Cédric Deluz, Ghérici Hassaïne, Menno Berend Tol

Elsevier Science Bv2014

Thermal Unfolding of a Mammalian Pentameric Ligand-gated Ion Channel Proceeds at Consecutive, Distinct Steps

Horst Vogel, Henning Paul-Julius Stahlberg, Cédric Deluz, Ghérici Hassaïne, Menno Berend Tol

Pentameric ligand-gated ion channels (LGICs) play an important role in fast synaptic signal transduction. Binding of agonists to the beta-sheet-structured extracellular domain opens an ion channel in the transmembrane alpha-helical region of the LGIC. How ...

Amer Soc Biochemistry Molecular Biology Inc2013

Large scale expression and purification of the mouse 5-HT3 receptor

Horst Vogel, Cédric Deluz, Menno Berend Tol, Ghérici Hassaïne

Receptors of the Cys-loop family are central to neurotransmission and primary therapeutic targets. In order to decipher their gating and modulation mechanisms, structural data is essential. However, structural studies require large amounts of pure, functio ...

Elsevier Science Bv2013

A Biochemical and Biophysical Investigation of 5-HT3 Receptor Stability

Menno Berend Tol

The 5-hydroxytryptamine 3 receptor (5-HT3R) is a member of the pentameric ligand-gated ion channel (pLGIC) family, that plays an important role in fast signal transduction and cell-cell communication in synapses: They convert a chemical signal from a neuro ...

EPFL2012