Publications de Marc Samuel Friedli

Pharmacological induction of a progenitor state for the efficient expansion of primary human hepatocytes

Didier Trono, Matthias Lütolf, Evaristo Jose Planet Letschert, Marc Samuel Friedli, Nathalie Brandenberg, Marco Cassano, Carmen Maria Unzu Ezquerro

The liver is an organ with strong regenerative capacity, yet primary hepatocytes have a low amplification potential in vitro, a major limitation for the cell-based therapy of liver disorders and for ex vivo biological screens. Induced pluripotent stem cell ...

2019

Method for preparing induced hepatic progenitor cells

Didier Trono, Carmen Maria Unzu Ezquerro, Marc Samuel Friedli

The present invention relates to a method for preparing induced hepatic progenitor cells (iHPC) comprising the steps of dedifferentiation of hepatocytes by culture with a culture medium comprising at least one activator of the Wnt signaling, Basic fibrobla ...

2017

Molecular Criteria for Defining the Naive Human Pluripotent State

Didier Trono, Evaristo Jose Planet Letschert, Julien Paul André Pontis, Julien Léonard Duc, Alexandra Iouranova, Marc Samuel Friedli, Michaël Imbeault, Haoyi Wang

Recent studies have aimed to convert cultured human pluripotent cells to a naive state, but it remains unclear to what extent the resulting cells recapitulate in vivo naive pluripotency. Here we propose a set of molecular criteria for evaluating the naive ...

Cell Press2016

Method for assessing the quality of various cells including induced pluripotent stem cells

Didier Trono, Marc Samuel Friedli, Carmen Maria Unzu Ezquerro

The present invention relates to a method for assessing the quality, utility and applicability of a cell according to the said method comprising the following steps: a) analyzing the expression of the TEs of said cell in order to set up an expression profi ...

2016

Potential Of Induced Pluripotent Stem Cells For The Treatment Of Crigler-Najjar Liver Disease

Didier Trono, Sandra Eloise Kjeldsen, Marc Samuel Friedli

Elsevier Science Bv2015

Loss of transcriptional control over endogenous retroelements during reprogramming to pluripotency

Didier Trono, Priscilla Turelli, Bastien Mangeat, Evaristo Jose Planet Letschert, Gabriela Ecco, Marc Samuel Friedli, Adamantia Kapopoulou, Benjamin Rauwel, Helen Mary Rowe, Carmen Maria Unzu Ezquerro

Endogenous retroelements (EREs) account for about half of the mouse or human genome, and their potential as insertional mutagens and transcriptional perturbators is suppressed by early embryonic epigenetic silencing. Here, we asked how ERE control is maint ...

Cold Spring Harbor Lab Press, Publications Dept2014

De novo DNA methylation of endogenous retroviruses is shaped by KRAB-ZFPs/KAP1 and ESET

Didier Trono, Sandra Eloise Kjeldsen, Sonia Verp, Marc Samuel Friedli, Helen Mary Rowe

Endogenous retroviruses (ERVs) undergo de novo DNA methylation during the first few days of mammalian embryogenesis, although the factors that control the targeting of this process are largely unknown. We asked whether KAP1 (KRAB-associated protein 1) is i ...

Company Of Biologists Ltd2013

Characterization of mouse Dactylaplasia mutations: a model for human ectrodactyly SHFM3

Denis Duboule, Marc Samuel Friedli

SHFM3 is a limb malformation characterized by the absence of central digits. It has been shown that this condition is associated with tandem duplications of about 500 kb at 10q24. The Dactylaplasia mice display equivalent limb defects and the two correspon ...

2008