Publications de Michael Werner

Microfluidic Single-Cell Analysis with Affinity Beads

Horst Vogel, Rudolf Hovius, Michael Werner, Loïc Arvind Roger Arm

A micrometer-sized affinity bead (red) is (i) taken up into a cell by phagocytosis, (ii) photochemically released from phagosomes, (iii) optically trapped by the cell, and (iv) isolated by cell lysis for subsequent analysis of captured intracellular analyt ...

2015

Use of the mitochondria toxicity assay for quantifying the viable cell density of microencapsulated jurkat cells

Jutta Christine Wandrey, Redouan Mahou, Michael Werner

The mitochondria toxicity assay (MTT assay) is an established method for monitoring cell viability based on mitochondrial activity. Here the MTT assay is proposed for the in situ quantification of the living cell density of microencapsulated Jurkat cells. ...

Wiley-Blackwell2013

Introducing the CTA concept

Mathieu Ribordy, Steve Couturier, Thomas Günther Bretz, Werner Lustermann, Christophe Bauer, Francisco Sanchez, Xiaoqing Zhou, Reto Frei, Etienne Antoine Julien Moulin, Michael Werner

The Cherenkov Telescope Array (CTA) is a new observatory for very high-energy (VHE) gamma rays. CTA has ambitions science goals, for which it is necessary to achieve full-sky coverage, to improve the sensitivity by about an order of magnitude, to span abou ...

Elsevier Science Bv2013

Downscaling the Analysis of Complex Transmembrane Signaling Cascades to Closed Attoliter Volumes

Horst Vogel, Rudolf Hovius, Luigino Grasso, Romain Wyss, Ghérici Hassaïne, Joachim Piguet, Michael Werner

Cellular signaling is classically investigated by measuring optical or electrical properties of single or populations of living cells. Here we show that ligand binding to cell surface receptors and subsequent activation of signaling cascades can be monitor ...

Public Library of Science2013

Microfluidic single cell analysis with affinity beads

Horst Vogel, Rudolf Hovius, Michael Werner, Loïc Arvind Roger Arm

Springer2013

Individual particle handling in a microfluidic system based on parallel laser trapping

René Salathé, Michael Werner, Horst Vogel, Bastien Rachet, Peter Ryser, Mathieu Grossenbacher

We present an optical trapping system combining individually addressable multiple laser traps with fluorescence spectroscopy. An in-line set of 64 near-IR laser diodes is used to create a line of individually addressable traps inside a microfluidic chip. T ...

Optical Society of America2011

Optical Trapping in Microfluidic Channels for Functional and Chemical Analysis of Single Cells

Michael Werner

The physiological properties of cells are typically investigated in ensembles yielding averaged data that mask heterogeneities present in any cell population. Single-cell analysis techniques based on microfluidic "lab on a chip" (LOC) devices have recently ...

EPFL2011

Microfluidic array cytometer based on refractive optical tweezers for parallel trapping, imaging and sorting of individual cells

René Salathé, Horst Vogel, Fabrice Merenda, Joachim Piguet, Michael Werner

Analysis of genetic and functional variability in populations of living cells requires experimental techniques capable of monitoring cellular processes such as cell signaling of many single cells in parallel while offering the possibility to sort interesti ...

Royal Society of Chemistry2011