Publications de Patrick Alain Sandoz

Dynamics of CLIMP-63 S-acylation control ER morphology

Vassily Hatzimanikatis, Françoise Gisou van der Goot Grunberg, Graham Knott, Kathryn Hess Bellwald, Laurence Gouzi Abrami, Béatrice Kunz, Luciano Andres Abriata, Sylvia Ho, Catherine Maclachlan, Patrick Alain Sandoz

A key player in the formation of endoplasmic reticulum sheets is CLIMP-63, but mechanistic details remained elusive. Here authors combined cellular experiments and mathematical modelling to show that S-acylation of CLIMP-63 regulates its function by mediat ...

Nature Portfolio2023

Palmitoylated acyl protein thioesterase APT2 deforms membranes to extract substrate acyl chains

Françoise Gisou van der Goot Grunberg, Matteo Dal Peraro, Laurence Gouzi Abrami, Florence Pojer, Maria Josefina Marcaida Lopez, Sylvia Ho, Francisco De Magalhães Sarmento R De Mesquita, Muhammad Umair Anwar, Patrick Alain Sandoz, Martina Audagnotto

Acyl protein thioesterase APT2 interacts with membranes via its charged beta-tongue, becomes palmitoylated by ZDHHC3/7 and deforms the bilayer to extract substrate acyl chains. APT2 deacylation leads to its membrane release and degradation. ...

NATURE RESEARCH2021

Image-based analysis of living mammalian cells using label-free 3D refractive index maps reveals new organelle dynamics and dry mass flux

Françoise Gisou van der Goot Grunberg, Christopher Claude Giuseppe Tremblay, Patrick Alain Sandoz

Holo-tomographic microscopy (HTM) is a label-free microscopy method reporting the fine changes of a cell’s refractive indices (RIs) in three dimensions at high spatial and temporal resolution. By combining HTM with epifluorescence, we demonstrate that mamm ...

2019

Active and dynamic mitochondrial S-depalmitoylation revealed by targeted fluorescent probes

Françoise Gisou van der Goot Grunberg, Patrick Alain Sandoz, Maria Eugenia Zaballa

Nature Research2018

Regulation of Endoplasmic Reticulum Architecture by Protein S-Palmitoylation

Patrick Alain Sandoz

The endoplasmic reticulum (ER) is the largest organelle in mammalian cells. More than 50 years ago, its morphology was already described as consisting of two major compartments: the rough ER and the smooth ER, which both fulfil precise and distinct functio ...

EPFL2017

Model-Driven Understanding of Palmitoylation Dynamics: Regulated Acylation of the Endoplasmic Reticulum Chaperone Calnexin

Vassily Hatzimanikatis, Françoise Gisou van der Goot Grunberg, Laurence Gouzi Abrami, Georgios Savoglidis, Tiziano Dallavilla, Patrick Alain Sandoz

Cellular functions are largely regulated by reversible post-translational modifications of proteins which act as switches. Amongst these, S-palmitoylation is unique in that it confers hydrophobicity. Due to technical difficulties, the understanding of this ...

Public Library of Science2016

New Insight into the Catalytic and Inhibition Mechanism of the Human Acyl Protein Thioesterase

Françoise Gisou van der Goot Grunberg, Martina Audagnotto, Matteo Dal Peraro, Sylvia Ho, Patrick Alain Sandoz, Nicole Andenmatten

Cell Press2016

Inertial microfluidic programming of microparticle-laden flows for solution transfer around cells and particles

Patrick Alain Sandoz, Léo Hamed Amini

Control of particles/cells and the surrounding fluid is enabling toward the purification of complex cellular samples, which still remains a bottleneck for point-of-care diagnostic devices. We explore a newly developed approach to engineer fluid stream moti ...

Springer Heidelberg2015

How many lives does CLIMP-63 have?

Françoise Gisou van der Goot Grunberg, Patrick Alain Sandoz

In 1995, in the Biochemical Society Transactions, Mundy published the first review on CLIMP-63 (cytoskeletonlinking membrane protein 63) or CKPA4 (cytoskeleton-associated protein 4), initially just p63 [1]. Here we review the following 20 years of research ...

2015

Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging

Theo Lasser, Françoise Gisou van der Goot Grunberg, Marcel Leutenegger, Noelia Laura Bocchio, Aurélien Godinat, Patrick Alain Sandoz, Azat Sharipov, Stefan Geissbuehler

Super-resolution optical fluctuation imaging (SOFI) provides an elegant way of overcoming the diffraction limit in all three spatial dimensions by computing higher-order cumulants of image sequences of blinking fluorophores acquired with a classical widefi ...

Nature Research2014