Publications de Paolo Annibale

Revealing GPCR oligomerization at the single-molecule level through a nanoscopic lens: methods, dynamics and biological function

Aleksandra Radenovic, Paolo Annibale, Marco Scarselli

The introduction of super-resolution fluorescence microscopy has allowed the visualization of single proteins in their biological environment. Recently, these techniques have been applied to determine the organization of class A G-protein-coupled receptors ...

Wiley-Blackwell2015

Progress in quantitative single-molecule localization microscopy

Aleksandra Radenovic, Paolo Annibale, Hendrik Georges Deschout, Marco Scarselli, Arun Shivanandan

With the advent of single-molecule localization microscopy (SMLM) techniques, intracellular proteins can be imaged at unprecedented resolution with high specificity and contrast. These techniques can lead to a better understanding of cell functioning, as t ...

Springer2014

Enlightening G-protein-coupled receptors on the plasma membrane using super-resolution photoactivated localization microscopy

Aleksandra Radenovic, Paolo Annibale, Marco Scarselli

The possibility to visualize and image the arrangement of proteins within the cell at the molecular level has always been an attraction for scientists in biological research. In particular, for signalling molecules such as GPCRs (G-protein-coupled receptor ...

Portland Press2013

Investigating the Impact of Single Molecule Fluorescence Dynamics on Photo Activated Localization Microscopy Experiments

Paolo Annibale

When fluorophores are densely packed in a biological sample, localizing them one at a time is one of the paths to optical super-resolution. Photo Activated Localization Microscopy (PALM) is one of the methods of this burgeoning field that presents a large ...

EPFL2012

Identification of the factors affecting co-localization precision for quantitative multicolor localization microscopy

Aleksandra Radenovic, Paolo Annibale, Marco Scarselli, Mattia Greco

This work explores the potential of multi-color Photoactivated Localization Microscopy (PALM) imaging to probe sub-diffraction limit interactions between proteins with spectrally separated labels. Using a PALM setup built around a commercial microscope axi ...

SpringerOpen2012

Identification of clustering artifacts in photoactivated localization microscopy

Ursula Röthlisberger, Aleksandra Radenovic, Stefano Vanni, Paolo Annibale, Marco Scarselli

Nature Publishing Group2011

ssDNA Binding Reveals the Atomic Structure of Graphene

Aleksandra Radenovic, Andras Kis, Paolo Annibale, Floriano Traversi

We used AFM to investigate the interaction of polyelectrolytes such as ssDNA and dsDNA molecules with graphene as a substrate. Graphene is an appropriate substrate due to its planarity, relatively large surfaces that are detectable via an optical microscop ...

American Chemical Society2010

Photoactivatable Fluorescent Protein mEos2 Displays Repeated Photoactivation after a Long-Lived Dark State in the Red Photoconverted Form

Aleksandra Radenovic, Paolo Annibale, Marco Scarselli

Illumination with 405 nm light can recover the emission for single green fluorescent protein (GFP) mutants that have gone into a long-lived dark state. The reported behavior is the standard for the reverse photoswitchable protein Dronpa and its mutants. Ho ...

2010