Personne
Mako Kamiya
Cette personne n’est plus à l’EPFL
Publications associées (4)
Low temperature and mTOR inhibition favor stem cell maintenance in human keratinocyte cultures
Kai Johnsson, Yann Barrandon, Johannes Alexander Mosig, Thomas Michael Braschler, Ariane Rochat, Jean-Baptiste Bureau, Fahd Azzabi Zouraq, Mako Kamiya
Adult autologous human epidermal stem cells can be extensively expanded ex vivo for cell and gene therapy. Identifying the mechanisms involved in stem cell maintenance and defining culture conditions to maintain stemness is critical, because an inadequate ...
WILEY2023Activatable fluorescent probes for hydrolase enzymes based on coumarin-hemicyanine hybrid fluorophores with large Stokes shifts
We show that the equilibrium of intramolecular spirocyclization of coumarin-hemicyanine hybrid fluorophores can be finely tuned by means of chemical modifications. We used this scaffold to develop activatable fluorescent probes with large Stokes shifts for ...
ROYAL SOC CHEMISTRY2020Labelling cell structures and tracking cell lineage in zebrafish using SNAP-tag
Kai Johnsson, Sambashiva Banala, Mako Kamiya
We present a method for the specific labelling of fusion proteins with synthetic fluorophores in Zebrafish. The method uses the SNAP-tag technology and O-6-benzylguanine derivatives of various synthetic fluorophores. We demonstrate how the method can be us ...
Wiley-Blackwell2011Localizable and highly sensitive calcium indicator based on a BODIPY fluorophore
We introduce here a new class of BODIPY-based Ca2+ indicators which can be derivatized with biological ligands that permit the localization of the indicators in living cells. The underivatized BODIPY-based Ca2+ indicator (BOCA-1) shows a 250-fold increase ...
American Chemical Society2010