Publications de Nora Guidotti | EPFL Graph Search

Tubulin engineering by semi-synthesis reveals that polyglutamylation directs detyrosination

Pierre Gönczy, Beat Fierz, Luc Reymond, Georgios Hatzopoulos, Cédric Pourroy, Po-Han Chang, Nora Guidotti, Ninad Dilip Agashe, Timothy Matthias Reichart, Eduard Hubert Theodoor Marius Ebberink

Microtubules, a critical component of the cytoskeleton, carry post-translational modifications (PTMs) that are important for the regulation of key cellular processes. Long-lived microtubules, in neurons particularly, exhibit both detyrosination of a-tubuli ...

NATURE PORTFOLIO2023

Multivalent Peptide Ligands To Probe the Chromocenter Microenvironment in Living Cells

Beat Fierz, Pablo Rivera Fuentes, Nora Guidotti, Maxime Nicolas Mivelaz, Adam Miklos Eördögh

Chromatin is spatially organized into functional states that are defined by both the presence of specific histone post-translational modifications (PTMs) and a defined set of chromatin-associated “reader” proteins. Different models for the underlying mecha ...

2022

SUV39 SET domains mediate crosstalk of heterochromatic histone marks

Beat Fierz, Sinan Kilic, Nora Guidotti

Y The SUV39 class of methyltransferase enzymes deposits histone H3 lysine 9 di- and trimethylation (H3K9me2/3), the hallmark of constitutive heterochromatin. How these enzymes are regulated to mark specific genomic regions as heterochromatic is poorly unde ...

eLIFE SCIENCES PUBL LTD2021

Development of chemical biology tools to reveal the function and organization of the silenced chromatin state

Nora Guidotti

Effector proteins are recruited to chromatin via transient interactions between their reader domains and histone post-translational modification (PTM) patterns. These interactions form signaling pathways that control gene expression or repression and which ...

EPFL2020

A Modular Ligation Strategy for Asymmetric Bivalent Nucleosomes Trimethylated at K36 and K27

Beat Fierz, Nora Guidotti, Andreas Linus Bachmann, Carolin Christine Lechner

In nature, individual histones in the same nucleosome can carry identical (symmetric) or different (asymmetric) post-translational modification (PTM) patterns, increasing the combinatorial complexity. Embryonic stem cells exhibit bivalent nucleosomes, some ...

WILEY-V C H VERLAG GMBH2019

"Doubly Orthogonal" Labeling of Peptides and Proteins

Jérôme Waser, Beat Fierz, Nora Guidotti, Raphaël Michel Henri Simonet-Davin, Romain Gaëtan Tessier

Herein, we report a cysteine bioconjugation methodology for the introduction of hypervalent iodine compounds onto biomolecules. Ethynylbenziodoxolones (EBXs) engage thiols in small organic molecules and cysteine-containing peptides and proteins in a fast a ...

2019

Engineered Multivalent Sensors to Detect Coexisting Histone Modifications in Living Stem Cells

Bart Deplancke, Beat Fierz, Horst Pick, David Michael Suter, Cédric Deluz, Aurore Marie-France Delachat, Antonio Carlos Alves Meireles Filho, Nora Guidotti, Andreas Linus Bachmann, Carolin Christine Lechner

2018