Publications de Yuhei Takado | EPFL Graph Search

Association between Brain and Plasma Glutamine Levels in Healthy Young Subjects Investigated by MRS and LC/MS

Both glutamine (Gln) and glutamate (Glu) are known to exist in plasma and brain. However, despite the assumed relationship between brain and plasma, no studies have clarified the association between them. Proton magnetic resonance spectroscopy (MRS) was se ...

2019

Hyperpolarized C-13 Magnetic Resonance Spectroscopy Reveals the Rate-Limiting Role of the Blood-Brain Barrier in the Cerebral Uptake and Metabolism of L-Lactate in Vivo

Bernard Lanz, Mor-Miri Mishkovsky, Tian Cheng, Arnaud Comment, Josefina Adriana Maria Bastiaansen, Hikari Ananda Infinity Yoshihara, Sylvain Eric Lengacher, Yuhei Takado

The dynamics of L-lactate transport across the blood-brain barrier (BBB) and its cerebral metabolism are still subject to debate. We studied lactate uptake and intracellular metabolism in the mouse brain using hyperpolarized C-13 magnetic resonance spectro ...

2018

Direct Monitoring of γ-Glutamyl Transpeptidase Activity In Vivo Using a Hyperpolarized (13) C-Labeled Molecular Probe

Arnaud Comment, Josefina Adriana Maria Bastiaansen, Hikari Ananda Infinity Yoshihara, Yuhei Takado, Emine Can

The γ-glutamyl transpeptidase (GGT) enzyme plays a central role in glutathione homeostasis. Direct detection of GGT activity could provide critical information for the diagnosis of several pathologies. We propose a new molecular probe, γ-Glu-[1-(13) C]Gly, ...

Wiley-Blackwell2016

Imaging the time-integrated cerebral metabolic activity with subcellular resolution through nanometer-scale detection of biosynthetic products deriving from (13)C-glucose

Anders Meibom, Graham Knott, Arnaud Comment, Stéphane Laurent Escrig, Yuhei Takado, Mojgan Masoodi

Glucose is the primary source of energy for the brain but also an important source of building blocks for proteins, lipids, and nucleic acids. Little is known about the use of glucose for biosynthesis in tissues at the cellular level. We demonstrate that l ...

Elsevier2015

Imaging liver and brain glycogen metabolism at the nanometer scale

Anders Meibom, Graham Knott, Arnaud Comment, Stéphane Laurent Escrig, Yuhei Takado, Mojgan Masoodi

In mammals, glycogen synthesis and degradation are dynamic processes regulating blood and cerebral glucose-levels within a well-defined physiological range. Despite the essential role of glycogen in hepatic and cerebral metabolism, its spatiotemporal distr ...

Elsevier Science Bv2015

Hyperpolarized 13C lactate as a substrate for in vivo metabolic studies in skeletal muscle

Rolf Gruetter, Arnaud Comment, Josefina Adriana Maria Bastiaansen, Hikari Ananda Infinity Yoshihara, Yuhei Takado

Resting skeletal muscle has a preference for the oxidation of lipids compared to carbohydrates and a shift towards carbohydrate oxidation is observed with increasing exercise. Lactate is not only an end product in skeletal muscle but also an important meta ...

2014

Hyperpolarized 13C lactate as a substrate for in vivo metabolic studies in skeletal muscle

Rolf Gruetter, Arnaud Comment, Josefina Adriana Maria Bastiaansen, Hikari Ananda Infinity Yoshihara, Yuhei Takado

2014

Over 35% liquid-state 13C polarization via dissolution dynamic nuclear polarization at 7 T and 1 K with ubiquitous nitroxyl radicals

Andrea Capozzi, Tian Cheng, Arnaud Comment, Yuhei Takado, Riccardo Balzan

The most versatile method to increase liquid-state 13C NMR sensitivity is dissolution dynamic nuclear polarization. The use of trityl radicals is usually required to obtain very large 13C polarization via this technique. We herein demonstrate that up to 35 ...

Royal Soc Chemistry2013

Hyperpolarized [1-13C]lactate metabolism in rat skeletal muscle in vivo

Rolf Gruetter, Arnaud Comment, Josefina Adriana Maria Bastiaansen, Hikari Ananda Infinity Yoshihara, Yuhei Takado

2013

Over 35% of liquid-state 13C polarization enhanced by TEMPOL via high field dissolution dynamic nuclear polarization

Andrea Capozzi, Tian Cheng, Arnaud Comment, Yuhei Takado, Riccardo Balzan

2013