Publications de Daniel Pawel Szlag

Imaging of cortical structures and microvasculature using extended-focus optical coherence tomography at 1.3 mu m

Theo Lasser, Paul James Marchand, Arno Pino Bouwens, Daniel Pawel Szlag, Damien Nguyen

2018

In vivo high-resolution cortical imaging with extended-focus optical coherence microscopy in the visible-NIR wavelength range

Theo Lasser, Paul James Marchand, Arno Pino Bouwens, Daniel Pawel Szlag

SPIE Society of Photo-optical Instrumentation Engineers2018

Massively parallel data processing for quantitative total flow imaging with optical coherence microscopy and tomography

Theo Lasser, Paul James Marchand, Daniel Pawel Szlag, Marcin Antoni Sylwestrzak

We present an application of massively parallel processing of quantitative flow measurements data acquired using spectral optical coherence microscopy (SOCM). The need for massive signal processing of these particular datasets has been a major hurdle for m ...

Elsevier2017

Label-free three-dimensional imaging of Caenorhabditis elegans with visible optical coherence microscopy

Johan Auwerx, Theo Lasser, Laurent Mouchiroud, Paul James Marchand, Arno Pino Bouwens, Vincenzo Sorrentino, Séverine Coquoz, Daniel Pawel Szlag

Fast, label-free, high-resolution, three-dimensional imaging platforms are crucial for high-throughput in vivo time-lapse studies of the anatomy of Caenorhabditis elegans, one of the most commonly used model organisms in biomedical research. Despite the ne ...

Public Library of Science2017

Visible spectrum extended-focus optical coherence microscopy for label-free sub-cellular tomography

Theo Lasser, Paul James Marchand, Arno Pino Bouwens, Adrien Charles-François Raymond Descloux, Daniel Pawel Szlag, David Nguyen, Jérôme Extermann, Miguel Sison, Séverine Coquoz

We present a novel extended-focus optical coherence microscope (OCM) attaining 0.7 μm axial and 0.4 μm lateral resolution maintained over a depth of 40 μm, while preserving the advantages of Fourier domain OCM. Our system uses an ultra-broad spectrum from ...

2017

Superresolved synthetic aperture microscope

Theo Lasser, Daniel Pawel Szlag, Tomas Lukes, Adrien Charles-François Raymond Descloux

Optical system comprising a synthetic aperture generating unit; said synthetic aperture generating unit being adapted for generating several apertures, which are correlated by interference for improving contrast and/or resolution based on the interference ...

2017

Statistical parametric mapping of stimuli evoked changes in total blood flow velocity in the mouse cortex obtained with extended-focus optical coherence microscopy

Theo Lasser, Paul James Marchand, Vincent Khashayar Shamaei, Arno Pino Bouwens, Tristan Bolmont, Daniel Pawel Szlag, Jérôme Extermann, David Nguyen

Functional magnetic resonance (fMRI) imaging is the current gold-standard in neuroimaging. fMRI exploits local changes in blood oxygenation to map neuronal activity over the entire brain. However, its spatial resolution is currently limited to a few hundre ...

Optical Soc Amer2017

Longitudinal three-dimensional visualisation of autoimmune diabetes by functional optical coherence imaging

Theo Lasser, Martin Villiger, Anne Grapin-Botton, Arno Pino Bouwens, Corinne Berclaz, Daniel Pawel Szlag, Jérôme Extermann, Joan Goulley

Aims/hypothesis It is generally accepted that structural and functional quantitative imaging of individual islets would be beneficial to elucidate the pathogenesis of type 1 diabetes. We here introduce functional optical coherence imaging (FOCI) for fast, ...

Springer Verlag2016

Label-free fast 3D coherent imaging reveals pancreatic islet micro-vascularization and dynamic blood flow

Theo Lasser, Paul James Marchand, Anne Grapin-Botton, Arno Pino Bouwens, Corinne Berclaz, David Nguyen, Daniel Pawel Szlag, Jérôme Extermann, Julia Sophie Katarina Nilsson

In diabetes, pancreatic beta-cells play a key role. These cells are clustered within structures called islets of Langerhans inside the pancreas and produce insulin, which is directly secreted into the blood stream. The dense vascularization of islets of La ...

2016

Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions

Theo Lasser, Aleksandra Radenovic, Lely Feletti, Marcel Leutenegger, Daniel Pawel Szlag, Tomas Lukes, Hendrik Georges Deschout, Azat Sharipov

Live-cell imaging of focal adhesions requires a sufficiently high temporal resolution, which remains a challenge for super-resolution microscopy. Here we address this important issue by combining photoactivated localization microscopy (PALM) with super-res ...

Nature Publishing Group2016