Publications de Arkadiy Bazhin

A bioluminescent-based probe for in vivo non-invasive monitoring of nicotinamide riboside uptake reveals a link between metastasis and NAD plus metabolism

Elena Goun, Pavlo Khodakivskyi, Arkadiy Bazhin, Aleksey Yevtodiyenko, Tamara Maric, Georgy Mikhaylov

Nicotinamide riboside (NR) is a form of vitamin B3 and is one of the most studied compounds for the restoration of cellular NAD+ levels demonstrating clinical potential in many metabolic and age-related disorders. Despite its wide commercial availability a ...

ELSEVIER ADVANCED TECHNOLOGY2023

Mitochondrial copper depletion suppresses triple-negative breast cancer in mice

Elena Goun, Arkadiy Bazhin, Min Chen, Ji Qi

Depletion of mitochondrial copper, which shifts metabolism from respiration to glycolysis and reduces energy production, is known to be effective against cancer types that depend on oxidative phosphorylation. However, existing copper chelators are too toxi ...

NATURE RESEARCH2021

Noninvasive imaging and quantification of bile salt hydrolase activity: From bacteria to humans

Elena Goun, Pavlo Khodakivskyi, Arkadiy Bazhin, Aleksey Yevtodiyenko

The microbiome-produced enzyme bile salt hydrolase (BSH) plays a central role in human health, but its function remains unclear due to the lack of suitable methods for measuring its activity. Here, we have developed a novel optical tool based on ultrasensi ...

AMER ASSOC ADVANCEMENT SCIENCE2021

Portable bioluminescent platform for in vivo monitoring of biological processes in non-transgenic animals

Aurélien Godinat, Elena Goun, Pavlo Khodakivskyi, Arkadiy Bazhin, Aleksey Yevtodiyenko, Ghyslain Martial Alain Budin, Tamara Maric

Bioluminescent imaging (BLI) is one of the most powerful and widely used preclinical imaging modalities. However, the current technology relies on the use of transgenic luciferase-expressing cells and animals and therefore can only be applied to a limited ...

Nature Research2021

A bioluminescent probe for longitudinal monitoring of mitochondrial membrane potential

Riccardo Sinisi, Elena Goun, Arkadiy Bazhin, Ghyslain Martial Alain Budin, Tamara Maric

Mitochondrial membrane potential (Delta Psi(m)) is a universal selective indicator of mitochondrial function and is known to play a central role in many human pathologies, such as diabetes mellitus, cancer and Alzheimer's and Parkinson's diseases. Here, we ...

NATURE RESEARCH2020

Bioluminescent-based imaging and quantification of glucose uptake in vivo

Riccardo Sinisi, Elena Goun, Pavlo Khodakivskyi, Arkadiy Bazhin, Aleksey Yevtodiyenko, Tamara Maric, Georgy Mikhaylov

Glucose is a major source of energy for most living organisms, and its aberrant uptake is linked to many pathological conditions. However, our understanding of disease-associated glucose flux is limited owing to the lack of robust tools. To date, positron- ...

NATURE PUBLISHING GROUP2019

A Universal Assay for Aminopeptidase Activity and Its Application for Dipeptidyl Peptidase-4 Drug Discovery

Gerardo Turcatti, Marc Chambon, Julien Bortoli Chapalay, Elena Goun, Jonathan Patrice Vesin, Arkadiy Bazhin, James William Collins

Aminopeptidases, such as dipeptidyl peptidase 4 (DPP-4, CD26), are potent therapeutic targets for pharmacological interventions because they play key roles in many important pathological pathways. To analyze amino-peptidase activity in vitro (including hig ...

AMER CHEMICAL SOC2019

Novel probes for the non-invasive bioluminescence imaging of mitochondrial membrane potential and dipeptidylpeptidase-4 activity in living animals

Arkadiy Bazhin

Current thesis summarizes the work on development of novel bioluminescent probes for measurement of activity of dipeptidylpep-tidase-4 (DPP-4) and for quantification of mitochondrial membrane potential (MMP). DPP-4 enzyme plays critical role in develop-men ...

EPFL2018

Bioorthogonal chemistry in bioluminescence imaging

Aurélien Godinat, Elena Goun, Arkadiy Bazhin

Bioorthogonal chemistry has developed significant over the past few decades, to the particular benefit of molecular imaging. Bioluminescence imaging (BLI) along with other imaging modalities have significantly benefitted from this chemistry. Here, we revie ...

ELSEVIER SCI LTD2018