Publications de Roman Bulushev

Nanocapillaries combined with optical tweezers as a single molecule technique for studying DNA-protein complexes

Interactions of proteins with DNA are essential for carrying out DNA's biological functions and performing a cellular cycle. Such processes as DNA replication, expression and repair are performed by an organised action of various proteins. To better unders ...

EPFL2017

Single Molecule Localization and Discrimination of DNA–Protein Complexes by Controlled Translocation Through Nanocapillaries

Aleksandra Radenovic, Sebastian Maerkl, Sanjin Marion, Roman Bulushev, Sebastian James Davis, Ekaterina Emilova Petrova

Through the use of optical tweezers we performed controlled translocations of DNA-protein complexes through nanocapillaries. We used RNA polymerase (RNAP) with two binding sites on a 7.2 kbp DNA fragment and a dCas9 protein tailored to have five binding si ...

American Chemical Society (ACS)2016

Relevance of the Drag Force during Controlled Translocation of a DNA–Protein Complex through a Glass Nanocapillary

Aleksandra Radenovic, Sanjin Marion, Roman Bulushev

Combination of glass nanocapillaries with optical tweezers allowed us to detect DNA-protein complexes in physiological conditions. In this system, a protein bound to DNA is characterized by a simultaneous change of the force and ionic current signals from ...

American Chemical Society (ACS)2015

Electrochemical Reaction in Single Layer MoS2: nanopores opened atom by atom

Aleksandra Radenovic, Andras Kis, Duncan Thomas Lindsay Alexander, Martina Lihter, Michael Graf, Dumitru Dumcenco, Ke Liu, Jiandong Feng, Roman Bulushev, Daria Krasnozhon

Ultrathin nanopore membranes based on 2D materials have demonstrated ultimate resolution toward DNA sequencing. Among them, molybdenum disulfide (MoS2) shows long-term stability as well as superior sensitivity enabling high throughput performance. The trad ...

American Chemical Society (ACS)2015

Identification of single nucleotides in MoS2 nanopores

Aleksandra Radenovic, Andras Kis, Dumitru Dumcenco, Ke Liu, Jiandong Feng, Roman Bulushev, Sergey Khlybov

The size of the sensing region in solid-state nanopores is determined by the size of the pore and the thickness of the pore membrane, so ultrathin membranes such as graphene and single-layer molybdenum disulphide could potentially offer the necessary spati ...

Nature Publishing Group2015

Combination of Optical Tweezers with Nanocapillaries as System for Estimation of DNA/Ligand Interactions

Aleksandra Radenovic, Lorenz Jan Steinbock, Roman Bulushev

Cell Press2014

ComEA Is Essential for the Transfer of External DNA into the Periplasm in Naturally Transformable Vibrio cholerae Cells

Aleksandra Radenovic, Matteo Dal Peraro, Melanie Blokesch, Lorenz Jan Steinbock, Roman Bulushev, Patrick Martin Seitz, Hasan Pezeshgi Modarres

The DNA uptake of naturally competent bacteria has been attributed to the action of DNA uptake machineries resembling type IV pilus complexes. However, the protein(s) for pulling the DNA across the outer membrane of Gram-negative bacteria remain speculativ ...

Public Library Science2014

Probing the size of proteins with glass nanopores

Aleksandra Radenovic, Melanie Blokesch, Lely Feletti, Lorenz Jan Steinbock, Roman Bulushev, Swati Krishnan

Single molecule studies using nanopores have gained attention due to the ability to sense single molecules in aqueous solution without the need to label them. In this study, short DNA molecules and proteins were detected with glass nanopores, whose sensiti ...

Royal Soc Chemistry2014

Measurement of the Position-Dependent Electrophoretic Force on DNA in a Glass Nanocapillary

Aleksandra Radenovic, Lorenz Jan Steinbock, Roman Bulushev, Sergey Khlybov

The electrophoretic force on a single DNA molecule inside a glass nanocapillary depends on the opening size and varies with the distance along the symmetrical axis of the nanocapillary. Using optical tweezers and DNA-coated beads, we measured the stalling ...

American Chemical Society2014

Shrinking Nanocapillaries to Low Noise Nanopores for Single Molecule Detection

Aleksandra Radenovic, Lorenz Jan Steinbock, Roman Bulushev, Swati Krishnan

Cell Press2014