Publications de Miguel Sison | EPFL Graph Search

Coherent Imaging of Cellular Dynamics

Mitochondrial dynamics refers to the processes of fusion, fission, and transport that aid mitochondria in accomplishing their many roles; including ATP production, oxygen sensing, and homeostasis. Due to their involvement in numerous essential cellular act ...

EPFL2017

3D Time-lapse Imaging and Quantification of Mitochondrial Dynamics

Theo Lasser, Paul James Marchand, Jérôme Extermann, Miguel Sison, Antonio Lopez, Amir Jean Nahas

We present a 3D time-lapse imaging method for monitoring mitochondrial dynamics in living HeLa cells based on photothermal optical coherence microscopy and using novel surface functionalization of gold nanoparticles. The biocompatible protein-based biopoly ...

Nature Research2017

Visible spectrum extended-focus optical coherence microscopy for label-free sub-cellular tomography

Theo Lasser, Paul James Marchand, Arno Pino Bouwens, Adrien Charles-François Raymond Descloux, Daniel Pawel Szlag, David Nguyen, Jérôme Extermann, Miguel Sison, Séverine Coquoz

We present a novel extended-focus optical coherence microscope (OCM) attaining 0.7 μm axial and 0.4 μm lateral resolution maintained over a depth of 40 μm, while preserving the advantages of Fourier domain OCM. Our system uses an ultra-broad spectrum from ...

2017

NIR-emitting and photo-thermal active nanogold as mitochondria-specific probes

Theo Lasser, Jérôme Extermann, Miguel Sison

We report a bioinspired multifunctional albumin derived polypeptide coating comprising grafted poly(ethylene oxide) chains, multiple copies of the HIV TAT derived peptide enabling cellular uptake as well as mitochondria targeting triphenyl-phosphonium (TPP ...

Royal Soc Chemistry2017

Optical projection tomography for rapid whole mouse brain imaging

Theo Lasser, Dimitri Nestor Alice Van De Ville, Jessica Sordet, Paul James Marchand, Arielle Louise Planchette, David Nguyen, Miguel Sison, Jérôme Extermann, Antonio Lopez, Marcin Antoni Sylwestrzak

In recent years, three-dimensional mesoscopic imaging has gained significant importance in life sciences for fundamental studies at the whole-organ level. In this manuscript, we present an optical projection tomography (OPT) method designed for imaging of ...

Optical Soc Amer2017

High-speed phase-shifting common-path quantitative phase imaging with a piezoelectric actuator

Theo Lasser, Antonio Lopez, Miguel Sison, Séverine Coquoz, Amir Jean Nahas

We present a phase-shifting quantitative phase imaging technique providing high temporal and spatial phase stability and high acquisition speed. A piezoelectric microfabricated phase modulator allows tunable modulation frequencies up to the kHz range. Afte ...

SPIE Society of Photo-optical Instrumentation Engineers2016

Fluorescent Nanodiamond–Gold Hybrid Particles for Multimodal Optical and Electron Microscopy Cellular Imaging

Theo Lasser, Miguel Sison, Weina Liu

There is a continuous demand for imaging probes offering excellent performance in various microscopy techniques for comprehensive investigations. of cellular processes by more than, one technique. FluoreScent nanodianiond gold nanoparticles (END Au) consti ...

American Chemical Society (ACS)2016

Three dimensional time lapse imaging of live cell mitochondria with photothermal optical lock-in optical coherence microscopy (Conference Presentation)

Theo Lasser, Christophe Pache, Miguel Sison, Jérôme Extermann, Amir Jean Nahas

Spie-Int Soc Optical Engineering2016