Publications de Hendrik Georges Deschout

Imaging of Optically Active Defects with Nanometer Resolution

Aleksandra Radenovic, Jiandong Feng, Hendrik Georges Deschout

American Chemical Society (ACS)2018

Investigating Focal Adhesion Substructures by Localization Microscopy

Aleksandra Radenovic, Daniel Sage, Lely Feletti, Hendrik Georges Deschout

Cells rely on focal adhesions (FAs) to carry out a variety of important tasks, including motion, environmental sensing, and adhesion to the extracellular matrix. Although attaining a fundamental characterization of FAs is a compelling goal, their extensive ...

Elsevier2017

Combining PALM and SOFI for quantitative imaging of focal adhesions in living cells

Theo Lasser, Aleksandra Radenovic, Lely Feletti, Tomas Lukes, Hendrik Georges Deschout, Azat Sharipov

Focal adhesions are complicated assemblies of hundreds of proteins that allow cells to sense their extracellular matrix and adhere to it. Although most focal adhesion proteins have been identified, their spatial organization in living cells remains challen ...

Spie-Int Soc Optical Engineering2017

Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions

Theo Lasser, Aleksandra Radenovic, Lely Feletti, Marcel Leutenegger, Daniel Pawel Szlag, Tomas Lukes, Hendrik Georges Deschout, Azat Sharipov

Live-cell imaging of focal adhesions requires a sufficiently high temporal resolution, which remains a challenge for super-resolution microscopy. Here we address this important issue by combining photoactivated localization microscopy (PALM) with super-res ...

Nature Publishing Group2016

Investigating Cellular Focal Adhesions on Nano-Patterned Substrates with Dual Color Photo-Activated Localization Microscopy

Aleksandra Radenovic, Hendrik Georges Deschout

Cell Press2015

High-Resolution Correlative Microscopy: Bridging the Gap between Single Molecule Localization Microscopy and Atomic Force Microscopy

Aleksandra Radenovic, Georg Fantner, Lely Feletti, Adrian Pascal Nievergelt, Pascal Damian Odermatt, Hendrik Georges Deschout, Arun Shivanandan, Radek Jankele

Nanoscale characterization of living samples has become essential for modern biology. Atomic force microscopy (AFM) creates topological images of fragile biological structures from biomolecules to living cells in aqueous environments. However, correlating ...

American Chemical Society (ACS)2015

On-chip light sheet illumination enables diagnostic size and concentration measurements of membrane vesicles in biofluids

Philippe Renaud, Sébastien Jiguet, Pietro Maoddi, Hendrik Georges Deschout

Cell-derived membrane vesicles that are released in biofluids, like blood or saliva, are emerging as potential non-invasive biomarkers for diseases, such as cancer. Techniques capable of measuring the size and concentration of membrane vesicles directly in ...

Royal Soc Chemistry2014

Progress in quantitative single-molecule localization microscopy

Aleksandra Radenovic, Paolo Annibale, Hendrik Georges Deschout, Marco Scarselli, Arun Shivanandan

With the advent of single-molecule localization microscopy (SMLM) techniques, intracellular proteins can be imaged at unprecedented resolution with high specificity and contrast. These techniques can lead to a better understanding of cell functioning, as t ...

Springer2014

Challenges in quantitative single molecule localization microscopy

Aleksandra Radenovic, Hendrik Georges Deschout, Marco Scarselli, Arun Shivanandan

Single molecule localization microscopy (SMLM), which can provide up to an order of magnitude improvement in spatial resolution over conventional fluorescence microscopy, has the potential to be a highly useful tool for quantitative biological experiments. ...

Elsevier2014