Publications de Marilisa Romito

Inner ear cellular imaging through scattering bone

Inner ear imaging is important for the assessment of hearing disorders. A major cause of hearing loss is the damage to the sensory hair cells, which are located inside the cochlea, a spiral-shaped bone in the inner ear. Imaging of intracochear hair cells i ...

SPIE-INT SOC OPTICAL ENGINEERING2019

Two-photon imaging and selective laser ablation of Cochlea hair cells through a multimode fiber probe

Demetri Psaltis, Christophe Moser, Damien Claude-Marie Loterie, Eirini Kakkava, Marilisa Romito

We demonstrate multimode fiber probe that accomodates dual modality properties for high power ultrashort pulse delivery. A commercially available multimode graded-index (GRIN) fiber is used for two-photon imaging and/or femtosecond laser ablation of Cochle ...

SPIE-INT SOC OPTICAL ENGINEERING2019

Biomedical optical techniques for intracochlear cellular imaging

Marilisa Romito

Imaging the inner ear microanatomy is of great importance for the assessment of the state of the cells responsible for sound detection. Hearing disorders are mainly due to a malfunction of the cochlea, the bone containing the cells inside the inner ear. Un ...

EPFL2019

Selective femtosecond laser ablation via two-photon fluorescence imaging through a multimode fiber

Demetri Psaltis, Christophe Moser, Damien Claude-Marie Loterie, Eirini Kakkava, Donald Benjaman Conkey, Marilisa Romito

We demonstrate the ability of a multimode fiber probe to provide two-photon fluorescence (TPF) imaging feedback that guides the femtosecond laser ablation (FLA) in biological samples for highly selective modifications. We implement the system through the p ...

OPTICAL SOC AMER2019

Imaging hair cells through laser-ablated cochlear bone

Demetri Psaltis, Ye Pu, Marilisa Romito

We report an innovative technique for the visualization of cells through an overlying scattering medium by combining femtosecond laser bone ablation and two-photon excitation fluorescence (TPEF) microscopy. We demonstrate the technique by imaging hair cell ...

2019

A device and method for increased light transmission through cochlear bone by laser ablation for in situ intracochlear imaging

Demetri Psaltis, Donald Benjaman Conkey, Marilisa Romito

A method for visualizing structures inside a cochlea, comprising the steps of irradiating a cochlear bone with a first laser light to ablate portions of the cochlear bone, to form a thinned area of the cochlear bone, and imaging intracochlear structures by ...

2017

Lensless two-photon imaging through a multicore fiber with coherence-gated digital phase conjugation

Demetri Psaltis, Christophe Moser, Nicolino Stasio, Donald Benjaman Conkey, Marilisa Romito, Edgar Emilio Morales Delgado

We performed near-diffraction limited two-photon fluorescence (TPF) imaging through a lensless, multicore-fiber (MCF) endoscope utilizing digital phase conjugation. The phase conjugation technique is compatible with commercially available MCFs with high co ...

SPIE Society of Photo-optical Instrumentation Engineers2016

Optofluidic Microstructures Containing Liquid Crystals

Andreas Vasdekis, Marilisa Romito, Luciano De Sio

We report our recent efforts on the realization and characterization of a conductive polydimethylsiloxane (PDMS) microstructure containing liquid crystals (LC). The sample has been realized by means of a layer by layer strategy. An indium tin oxide (ITO) l ...

Taylor & Francis Ltd2013

Electro-switchable polydimethylsiloxane-based optofluidics

Andreas Vasdekis, Marilisa Romito, Luciano De Sio

We report on the fabrication and characterization of a new generation of electro-switchable optofluidic devices based on flexible substrates, combined with the extraordinary properties of reconfigurable soft-materials. A conductive polydimethylsiloxane mic ...

Royal Soc Chemistry2012