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Tomas Lukes

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Publications associées (17)

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Quantifying protein densities on cell membranes using super-resolution optical fluctuation imaging

Theo Lasser, Tomas Lukes

Surface molecules, distributed in diverse patterns and clusters on cell membranes, influence vital functions of living cells. It is therefore important to understand their molecular surface organisation under different physiological and pathological condit ...

2017

Correcting for photodestruction in super-resolution optical fluctuation imaging

Theo Lasser, Arno Pino Bouwens, Tomas Lukes

Super-resolution optical fluctuation imaging overcomes the diffraction limit by analyzing fluctuations in the fluorophore emission. A key assumption of the imaging is that the fluorophores are independent, though this is invalidated in the presence of phot ...

Nature Publishing Group2017

Superresolved synthetic aperture microscope

Theo Lasser, Daniel Pawel Szlag, Tomas Lukes, Adrien Charles-François Raymond Descloux

Optical system comprising a synthetic aperture generating unit; said synthetic aperture generating unit being adapted for generating several apertures, which are correlated by interference for improving contrast and/or resolution based on the interference ...

2017

SIMToolbox: a MATLAB toolbox for structured illumination fluorescence microscopy

Tomas Lukes

SIMToolbox is an open-source, modular set of functions for MATLAB equipped with a user-friendly graphical interface and designed for processing two-dimensional and three-dimensional data acquired by structured illumination microscopy (SIM). Both optical se ...

Oxford University Press2016

SOFI Simulation Tool: A Software Package for Simulating and Testing Super-Resolution Optical Fluctuation Imaging

Theo Lasser, Marcel Leutenegger, Tomas Lukes, Azat Sharipov, Stefan Geissbuehler

Super-resolution optical fluctuation imaging (SOFI) allows one to perform sub-diffraction fluorescence microscopy of living cells. By analyzing the acquired image sequence with an advanced correlation method, i.e. a high-order cross-cumulant analysis, supe ...

Public Library of Science2016

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Live-cell imaging of focal adhesions requires a sufficiently high temporal resolution, which remains a challenge for super-resolution microscopy. Here we address this important issue by combining photoactivated localization microscopy (PALM) with super-res ...

Nature Publishing Group2016

We introduce and demonstrate a new high performance image reconstruction method for super-resolution structured illumination microscopy based on maximum a posteriori probability estimation (MAP-SIM). Imaging performance is demonstrated on a variety of fluo ...

Optical Society of America2014