Publications de Antonius Chrisnandy

Morphodynamics of human early brain organoid development

Brain organoids enable the mechanistic study of human brain development and provide opportunities to explore self-organization in unconstrained developmental systems1, 2–3. Here we establish long-term, live light-sheet microscopy on unguided brain organoid ...

Springer Science and Business Media LLC2025

An extracellular matrix niche secreted by epithelial cells drives intestinal organoid formation

Matthias Lütolf, Antonius Chrisnandy

Cell Press - Elsevier2025

Open-source microscope add-on for structured illumination microscopy

Theo Lasser, Matthias Lütolf, Aleksandra Radenovic, Andrew Charles Oates, Georg Fantner, John McKinney, Nathan Cyprien Arsène Ronceray, Olivier François Venzin, Antonius Chrisnandy, Prabhu Prasad Swain

Super-resolution techniques expand the abilities of researchers who have the knowledge and resources to either build or purchase a system. This excludes the part of the research community without these capabilities. Here we introduce the openSIM add-on to ...

Nature Portfolio2024

Collagen signaling and matrix stiffness regulate multipotency in glandular epithelial stem cells in mice

Matthias Lütolf, Antonius Chrisnandy, Saba Rezakhani

Glandular epithelia, including mammary gland (MG) and prostate, are composed of luminal and basal cells. During embryonic development, glandular epithelia arise from multipotent stem cells (SCs) that are replaced after birth by unipotent basal and unipoten ...

Nature Research2024

Engineering hydrogel microenvironments for epithelial organoid culture

Antonius Chrisnandy

Organoids, miniature tissues generated from self-organizing stem cells within three-dimensional (3D) extracellular matrices (ECM), have opened up exciting possibilities for in vitro studies of complex physiological processes. A key factor in the success of ...

EPFL2024

Dynamic Hydrogel Design For Spatiotemporal Control Of Morphogenesis

Matthias Lütolf, Nicolas Antoine Vincent Broguiere, Antonius Chrisnandy

MARY ANN LIEBERT, INC2023

Deterministic scRNA-seq captures variation in intestinal crypt and organoid composition

Matthias Lütolf, Bart Deplancke, Esther Amstad, Vincent Roland Julien Gardeux, Julie Marie Russeil, Rita Sarkis, Jörn Pezoldt, Johannes Julius Bues, Antonius Chrisnandy, Riccardo Dainese

DisCo is a deterministic droplet microfluidics tool for single-cell analysis on low cell input samples, which is demonstrated to profile individual intestinal organoids and in vivo-derived small tissues. ...

NATURE PORTFOLIO2022

Synthetic dynamic hydrogels promote degradation-independent in vitro organogenesis

Matthias Lütolf, Nicolas Antoine Vincent Broguiere, Antonius Chrisnandy, Saba Rezakhani, Delphine Lorraine Perle Blondel

Epithelial organoids are most efficiently grown from mouse-tumour-derived, reconstituted extracellular matrix hydrogels, whose poorly defined composition, batch-to-batch variability and immunogenicity limit clinical applications. Efforts to replace such il ...

NATURE PORTFOLIO2022