Publications de Georgios Hatzopoulos

Chemical Probe for Imaging of Polo-like Kinase 4 and Centrioles

Kai Johnsson, Pierre Gönczy, Luc Reymond, Georgios Hatzopoulos, Aleksandar Salim

Polo-like kinase (Plk4) is a serine/threonine-proteinkinase thatis essential for biogenesis of the centriole organelle and is enrichedat centrioles. Herein, we introduce Cen-TCO, a chemical probe basedon the Plk4 inhibitor centrinone, to image Plk4 and cen ...

AMER CHEMICAL SOC2023

Tubulin engineering by semi-synthesis reveals that polyglutamylation directs detyrosination

Pierre Gönczy, Beat Fierz, Luc Reymond, Georgios Hatzopoulos, Cédric Pourroy, Po-Han Chang, Nora Guidotti, Ninad Dilip Agashe, Timothy Matthias Reichart, Eduard Hubert Theodoor Marius Ebberink

Microtubules, a critical component of the cytoskeleton, carry post-translational modifications (PTMs) that are important for the regulation of key cellular processes. Long-lived microtubules, in neurons particularly, exhibit both detyrosination of a-tubuli ...

NATURE PORTFOLIO2023

CenFind: a deep-learning pipeline for efficient centriole detection in microscopy datasets

Pierre Gönczy, Georgios Hatzopoulos, Sahand Jamal Rahi, Martin Weigert

BackgroundHigh-throughput and selective detection of organelles in immunofluorescence images is an important but demanding task in cell biology. The centriole organelle is critical for fundamental cellular processes, and its accurate detection is key for a ...

BMC2023

Structures of SAS-6 coiled coil hold implications for the polarity of the centriolar cartwheel

Pierre Gönczy, Georgios Hatzopoulos

Centrioles are eukaryotic organelles that template the formation of cilia and flagella, as well as organize the microtubule network and the mitotic spindle in animal cells. Centrioles have proximal-distal polarity and a 9 fold radial symmetry imparted by a ...

CELL PRESS2022

Kinetic and structural roles for the surface in guiding SAS-6 self-assembly to direct centriole architecture

Pierre Gönczy, Georg Fantner, Georgios Hatzopoulos, Adrian Pascal Nievergelt, Niccolo Banterle, Santiago Harald Andany, Charlène Cécile Geneviève Brillard

Discovering mechanisms governing organelle assembly is a fundamental pursuit in biology. The centriole is an evolutionarily conserved organelle with a signature 9-fold symmetrical chiral arrangement of microtubules imparted onto the cilium it templates. Th ...

Nature Portfolio2021

Pulchelloid A, a sesquiterpene lactone from the Canadian prairie plant Gaillardia aristata inhibits mitosis in human cells

Pierre Gönczy, Georgios Hatzopoulos, Tatiana Favez

Background The Canadian prairie ecosystem presents a rich source of natural products from plants that are subjected to herbivory by grazing mammals. This type of ecological competition may contribute to the production of natural products of interest in cel ...

SPRINGER2021

Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly

Pierre Gönczy, Georg Fantner, Georgios Hatzopoulos, Tatiana Favez, Oliver Hantschel, Virginie Hamel, Niccolo Banterle, Santiago Harald Andany

Centrioles are evolutionarily conserved multi-protein organelles essential for forming cilia and centrosomes. Centriole biogenesis begins with self-assembly of SAS-6 proteins into 9-fold symmetrical ring polymers, which then stack into a cartwheel that sca ...

Nature Research2021

Novel features of centriole polarity and cartwheel stacking revealed by cryo-tomography

Pierre Gönczy, Davide Demurtas, Georgios Hatzopoulos, Sergey Nazarov, Alexandra Bezler, Paul Guichard, Veronika Nemcíková Villímová

Centrioles are polarized microtubule-based organelles that seed the formation of cilia, and which assemble from a cartwheel containing stacked ring oligomers ofSAS-6 proteins. A cryo-tomography map of centrioles from the termite flagellateTrichonymphaspp. ...

2020

Centriole assembly at a glance

Pierre Gönczy, Georgios Hatzopoulos

The centriole organelle consists of microtubules (MTs) that exhibit a striking 9-fold radial symmetry. Centrioles play fundamental roles across eukaryotes, notably in cell signaling, motility and division. In this Cell Science at a Glance article and accom ...

2019

The Human Centriolar Protein CEP135 Contains a Two-Stranded Coiled-Coil Domain Critical for Microtubule Binding

Pierre Gönczy, Paul Guichard, Georgios Hatzopoulos

Centrioles are microtubule-based structures that play important roles notably in cell division and cilium biogenesis. CEP135/Bld10p family members are evolutionarily conserved microtubule-binding proteins important for centriole formation. Here, we analyze ...

Elsevier2016