Publications de Barbora Lavickova

Improved Cell-Free Transcription-Translation Reactions in Microfluidic Chemostats Augmented with Hydrogel Membranes for Continuous Small Molecule Dialysis

Sebastian Maerkl, Laura Sophie Grasemann, Barbora Lavickova

Increasing the protein production capacity of the PURE cell-free transcription-translation (TX-TL) system will be key to implementing complex synthetic biological circuits, and to establishing a fully self-regenerating system as a basis for the development ...

AMER CHEMICAL SOC2022

Towards an artificial cell: Development of a system which self-regenerates the protein components of the PURE system in microfluidic reactors

Barbora Lavickova

Thanks to recent advancements in synthetic biology, the dream of creating a synthetic cell has become feasible. However, due to its inherent complexity, one of the fundamental functions of all living systems, i.e., self-replication, remains to be introduce ...

EPFL2021

Degradation study on molecules released from laser-based jet injector

Christophe Moser, Jan Krizek, Barbora Lavickova

Development of needle-free methods to administer injectable therapeutics has been researched for a few decades. We focused our attention on a laser-based jet injection technique where the liquid-jet actuation mechanism is based on optical cavitation. This ...

2021

OnePot PURE Cell-Free System

Sebastian Maerkl, Laura Sophie Grasemann, Barbora Lavickova

The defined PURE (protein synthesis using recombinant elements) transcription-translation system provides an appealing chassis for cell-free synthetic biology. Unfortunately, commercially available systems are costly, and their tunability is limited. In co ...

2021

A partially self-regenerating synthetic cell

Sebastian Maerkl, Barbora Lavickova, Nadanai Laohakunakorn

Self-regeneration is a fundamental function of all living systems. Here we demonstrate partial molecular self-regeneration in a synthetic cell. By implementing a minimal transcription-translation system within microfluidic reactors, the system is able to r ...

Nature Research2020

Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology

Sebastian Maerkl, Laura Sophie Grasemann, Zoe Newell Swank, Barbora Lavickova, Grégoire Michielin, Nadanai Laohakunakorn, Amir Shahein

Cell-free systems offer a promising approach to engineer biology since their open nature allows for well-controlled and characterized reaction conditions. In this review, we discuss the history and recent developments in engineering recombinant and crude e ...

2020

A Simple, Robust, and Low-Cost Method To Produce the PURE Cell Free System

Sebastian Maerkl, Barbora Lavickova

We demonstrate a simple, robust, and low-cost method for producing the PURE cell-free transcription translation system. Our OnePot PURE system achieved a protein synthesis yield of 156 peg/mL at a cost of 0.09 USD/ktL, leading to a 14-fold improvement in c ...

2019