Publications de Huijie Pan | EPFL Graph Search

The Function of Two Radical-SAM Enzymes, HcgA and HcgG, in the Biosynthesis of the [Fe]-Hydrogenase Cofactor

In the biosynthesis of the iron-guanylylpyridinol (FeGP) cofactor, 6-carboxymethyl-5-methyl-4-hydroxy-2-pyridinol (1) is 3-methylated to form 2, then 4-guanylylated to form 3, and converted into the full cofactor. HcgA-G proteins catalyze the biosynthetic ...

Wiley-VCH Verlag GmbH2022

In Vitro Biosynthesis of the [Fe]-Hydrogenase Cofactor Verifies the Proposed Biosynthetic Precursors

Xile Hu, Huijie Pan

In the FeGP cofactor of [Fe]-hydrogenase, low-spin Fe-II is in complex with two CO ligands and a pyridinol derivative; the latter ligates the iron with a 6-acylmethyl substituent and the pyridinol nitrogen. A guanylylpyridinol derivative, 6-carboxymethyl-3 ...

Wiley-VCH Verlag GmbH2022

Biomimetic Hydrogenation Catalyzed by a Manganese Model of [Fe]-Hydrogenase

Xile Hu, Huijie Pan

[Fe]‐hydrogenase is an efficient biological hydrogenation catalyst. Despite intense research, Fe complexes mimicking the active site of [Fe]‐hydrogenase have not achieved turnovers in hydrogenation reactions. Herein, we describe the design and development ...

Wiley-VCH Verlag GmbH2020

A catalytically active [Mn]-hydrogenase incorporating a non-native metal cofactor

Xile Hu, Farzaneh Fadaei Tirani, Matthew Wodrich, Huijie Pan

Nature carefully selects specific metal ions for incorporation into the enzymes that catalyse the chemical reactions necessary for life. Hydrogenases, enzymes that activate molecular H-2, exclusively utilize Ni and Fe in [NiFe]-, [FeFe]- and [Fe]-hydrogean ...

2019

Functional Models of the Nickel Pincer Nucleotide Cofactor of Lactate Racemase

Xile Hu, Farzaneh Fadaei Tirani, Matthew Wodrich, Huijie Pan, Renyi Shi

A novel nickel pincer cofactor was recently discovered in lactate racemase. Reported here are three synthetic nickel pincer complexes that are both structural and functional models of the pincer cofactor in lactate racemase. DFT computations suggest the ip ...

Wiley-VCH Verlag GmbH2019