Hugo José Rodriguez Franco | EPFL Graph Search

À propos de ce résultat

Cette page est générée automatiquement et peut contenir des informations qui ne sont pas correctes, complètes, à jour ou pertinentes par rapport à votre recherche. Il en va de même pour toutes les autres pages de ce site. Veillez à vérifier les informations auprès des sources officielles de l'EPFL.

Stabilizing Polymer Coatings Alter the Protein Corona of DNA Origami and Can Be Engineered to Bias the Cellular Uptake

Maartje Martina Cornelia Bastings, Hugo José Rodriguez Franco, Jorieke Weiden

With DNA-based nanomaterials being designed for applicationsincellular environments, the need arises to accurately understand theirsurface interactions toward biological targets. As for any materialexposed to protein-rich cell culture conditions, a protein coronawill establish around DNA nanoparticles, potentially altering thea-priori designed particle function. Here, we first set out to identifythe protein corona around DNA origami nanomaterials, taking into accountthe application of stabilizing block co-polymer coatings (oligolysine-1kPEGor oligolysine-5kPEG) widely used to ensure particle integrity. Byimplementing a label-free methodology, the distinct polymer coatingconditions show unique protein profiles, predominantly defined bydifferences in the molecular weight and isoelectric point of the adsorbedproteins. Interestingly, none of the applied coatings reduced thediversity of the proteins detected within the specific coronae. Wethen biased the protein corona through pre-incubation with selectedproteins and show significant changes in the cell uptake. Our studycontributes to a deeper understanding of the complex interplay betweenDNA nanomaterials, proteins, and cells at the bio-interface.

AMER CHEMICAL SOC2023

Strategic Insights into Engineering Parameters Affecting Cell Type-Specific Uptake of DNA-Based Nanomaterials

Maartje Martina Cornelia Bastings, Alice Comberlato, Marianna Mainardi Koga, Hugo José Rodriguez Franco

DNA-based nanomaterials are gaining popularity as uniform and programmable bioengineering tools as a result of recent solutions to their weak stability under biological conditions. The DNA nanotechnology platform uniquely allows decoupling of engineering parameters to comprehensively study the effect of each upon cellular encounter. We here present a systematic analysis of the effect of surface parameters of DNA-based nanoparticles on uptake in three different cell models: tumor cells, macrophages, and dendritic cells. The influence of surface charge, stabilizing coating, fluorophore types, functionalization technique, and particle concentration employed is found to cause significant differences in material uptake among these cell types. We therefore provide new insights into the large variance in cell type-specific uptake, highlighting the necessity of proper engineering and careful assay development when DNA-based materials are used as tools in bioengineering and as future nanotherapeutic agents.

AMER CHEMICAL SOC2022