Êtes-vous un étudiant de l'EPFL à la recherche d'un projet de semestre?
Travaillez avec nous sur des projets en science des données et en visualisation, et déployez votre projet sous forme d'application sur Graph Search.
We report here a simple method, based on electrostatic self-assembly, for realizing bead-based single protein microarrays. We generate positively-charged surface micro-patterns on a glass substrate and used them as the template for adsorbing negatively-charged carboxylated beads. The, positively-charged, template for bead adsorption is generated by patterning a aminosilane layer using conventional lift-off technique. The –COOH groups which are present on the patterned beads’ surface is activated using carbodiimide chemistry and then a protein of interest is covalently coupled to the bead surface. The patterned beads bind strongly to the aminosilane patterns and they are able to even withstand agitated rinse in de-ionized water. We also show here that the same method can be extended for bead patterning with in sealed microfluidic channels.
Pierre Gönczy, Georg Fantner, Adrian Pascal Nievergelt, Niccolo Banterle, Santiago Harald Andany