Glycoprotein-associated amino acid exchangers: broadening the range of transport specificity
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Membrane protein research depends largely on the availability of functional protein in an instrumental set-up for application of biophysical investigations. We used an in vitro synthesis strategy in the presence of a solid supported lipid membrane as a new ...
Tumor necrosis factor TNF can trigger increases in membrane conductance of mammalian cells in a receptor-independent manner via its lectin-like domain. A lectin-deficient TNF mutant, lacking this activity, was able to bind to artificial liposomes in a pH-d ...
Membrane proteins fulfill many central functions in the biological membrane. The insertion process of these proteins and their structure, which are intimately linked to their function, are not yet well understood. As a model we studied three proteins recon ...
Lipophilicity of compounds with therapeutic interest represents one of the most important physicochemical parameters in predicting and interpreting transport processes across biological barriers. This property is commonly measured as a drug | membrane part ...
Amino acid transport across cellular membranes is mediated by multiple transporters with overlapping specificities. We recently have identified the vertebrate proteins which mediate Na+-independent exchange of large neutral amino acids corresponding to tra ...
Aerolysin is one of a large group of bacterial proteins that can kill target cells by forming discrete channels in their plasma membranes. The toxin has many properties in common with the porins of the Gram-negative bacterial outer membrane, including an e ...
A review with 91 refs. The use of solid-state NMR as a tool to det. the structure of membrane mols. is reviewed with a particular emphasis on techniques that provide information on orientation or order. Expts. reported here have been performed in membranes ...
Conformational changes occurring upon membrane binding and subsequent insertion of staphylococcal alpha-toxin were studied using complementary spectroscopic techniques. Experimental conditions were established where binding could be uncoupled from membrane ...
A review with many refs. Ammonia has been reported to be toxic and inhibitory for mammalian cell cultures for many years. Redn. of growth rates and maximal cell densities in batch cultures, changes in metabolic rates, perturbation of protein processing and ...